非Smad通路在小鼠结肠癌肝转移中的作用-外科学(普通外科)专业论文.docxVIP

绪言 ··············································································· 50 材料和方法 ······································································ 52 结果 ··············································································· 54 讨论 ··············································································· 57 参考文献 ········································································· 58 综述 ··············································································· 61 TGF-β 与肿瘤 61 实验常用缓冲液、试剂配制方法 ··········································· 92 发表论文 ········································································· 96 致谢 ··············································································· 97 华中科技大学博士学位论文 华 中 科 技 大 学 博 士 学 位 论 文 PAGE 10 PAGE 10 缩略词表 ALK activin receptor-like kinase 激活素受体样激酶 BMP bone morphogenetic protein 骨形态发生蛋白 BSA bovine serum albumin 牛血清白蛋白 CCK-8 cell counting kit-8 细胞计数试剂盒-8 CDK cyclin dependent kinase 细胞周期蛋白依赖激酶 CRC colorectal cancer 结直肠癌 CTGF connective tissue growth factor 结缔组织生长因子 CTL cytotoxic T lymphocyte 细胞毒 T 淋巴细胞 DN dominant negative 显性负相 ECM extracellular matrix 细胞外基质 EDTA ethylenediamine tetra-acetate 乙二胺四乙酸盐 EMT Epithelial-to-mesenchymal transition 上皮细胞-间充质转化 ER estradiol receptor 雌二醇受体 ERK Extracellular signal-regulated kinase 细胞外信号调节激酶 FBS Fetal Bovine Serum 胎牛血清 FITC fluorescein isocyanate 异氰酸荧光素 GDP guanosine diphosphate 二磷酸鸟甘酸 GTP Guanosine Triphosphate 三磷酸鸟甘酸 HGF hepatocyte growth factor 肝细胞生长因子 IFN Interferon 干扰素 IL Interleukin 白介素 JNK c-jun N-terminal kinase c-Jun 氨基末端激酶 JPS Juvenile polyposis syndrome 幼年性息肉病综合征 KD Knockdown 敲减 MAPK Mitogen activated protein kinese 有丝分裂原活化蛋白激酶 MMP matrix metalloproteinase 基质金属蛋白酶 MMP-9 Matrix metalloproteinase-9 基质金属蛋白酶-9 MOI multiplicity of infection 复感染指数 mTOR mammalian target of rapamycin 钠巴霉素的哺乳动物靶点 NC negative control 阴性对照 NK cell nature killer cell 自然杀伤细胞 PBS phosphate buffered solution 磷酸盐缓冲溶液 PCR polymerase chain reaction 聚合酶链反应 PDGF p