气相色谱法检测饮料中二羰基化合物-食品科学.PDF

※安全检测 食品科学 2015, Vol.36, No.24 235 气相色谱法检测饮料中二羰基化合物 王 晨，李晓明，卢永翎，郑铁松，吕丽爽* （南京师范大学金陵女子学院，江苏南京 210097 ） 摘  要：建立同时在线检测丙酮醛和乙二醛的气相色谱方法。确定检测二羰基化合物的最佳条件为：以丁二酮为内 标，以邻苯二胺为衍生化试剂，邻苯二胺的用量67 倍于二羰基化合物、衍生化时间10 min、萃取溶剂二氯甲烷、超 15 min 2 40 5 /min 2.0 mL/min 1 1 声时间 、萃取 次、柱箱初始温度 ℃、程序升温 ℃ ，色谱柱载气流量 ，分流比 ∶。丙酮 R ≈10 0.06 mg/L 0.08 mg/L R ≈ 3 0.02 mg/L 0.03 mg/L 醛和乙二醛的定量限（ SN ）分别为 和 ，检出限（ SN ）分别为 和 ，方 法灵敏度高。 关键词：丙酮醛；乙二醛；气相色谱 Detection of Dicarbonyl Compounds in Beverages by Gas Chromatography WANG Chen, LI Xiaoming, LU Yongling, ZHENG Tiesong, LÜ Lishuang* (Ginling College, Nanjing Normal University, Nanjing 210097, China) Abstract: This study established an efficient method for the detection of methylglyoxal (MGO) and glyoxal (GO) with a gas chromatograph (GC). The optimum experimental conditions were set as follows: ratio of O-phenylenediamine to α-dicarbonyl , compounds,67;derivatizationtime 10min;extractionsolvent,methylenechloride;ultrasonic extractiontime, 15min;and the number of extraction, 2. The injector was operated in split mode with a split ratio of 1:1, and the carrier gas (helium) flow rate through the chromatographic column was 2.0 mL/min. The GC oven temperature was programmed as follows: the initial oven temperature was set as 40 and then increased at a rate of 5 /min. The minimum quantitation limits (R ℃ ℃