翻白草降糖降脂作用及机理初探实验研究-药理学专业论文.docx

摘要 摘要 摘要 摘要 PAGE PAGE IV III III 组 糖尿乐组(见图 4-8)；TG 水平较阳性对照组由低向高排列为：二甲双胍组 糖尿乐组 翻白草 95%乙醇提取物治疗组(见图 4-6)；HDL-C 显示出比模型组升 高，较阳性对照组由高向低排列为：糖尿乐 翻白草 95%乙醇提取物～二甲双 胍组(见图 4-7)。即生化指标检测翻白草提取物有明显的降糖、降低 TC 的作 用。治疗糖尿病效果与目前临床已用的两个中西药对比，弱于西药二甲双胍，明 显优于中药的糖尿乐效果。病理组织切片观察发现，DM 大鼠在翻白草治疗后， 胰岛细胞数目均有增多；但是各组大鼠心脏、肾脏和动脉等组织未见明显差异， 是因为动物造模时间较短，尚未造成组织的损伤所致。同时，翻白草给药组也未 出现明显异常，可以看出翻白草的毒副作用较小，属于一类较安全的中药。 本课题通过对翻白草不同提取物抗糖尿病的药效学研究，证明了翻白草具有 降糖降脂作用，而此作用可以被归属为翻白草提取物中固有的抗自由基的化学活 性成分的存在。该药理药效学研究，为翻白草的植物化学研究及进一步寻找抗糖 尿病新药提供了实验依据和理论指导。由此采用的抗糖尿病酪氨酸硝基化体外反 应模型，用于中草药抗糖尿病有效成分筛选，是本课题的研究的创新点。 关 键 词：翻白草；糖尿病；药效学；体外；体内 论文类型：基础研究 Subject: Original mechanism research on the antihyperglycaemic and antihyperlipidaetic activity of the Potentilla discolor Bunge Specialty: Pharmacology Name: Shao Fang-fang Supervisor: Yin Wei-ping ABSTRACT In recent years, Diabetes mellitus (DM) should be also pay more attention with the development of againsting serious illness study in domestic and international. According to dates provided by International Diabetes Federation, there are more than 250 million people with diabetes in global at present. If any active measures weren’t taken, this number will be increased to 380 million in twenty years. The study of Diabetes mellitus is as important as cancer and cardiovascular disease in China. This topic study for a pharmacological of Potentilla discolor Bunge. in vivo and vitro based on the folk herb medicine from Potentilla Discolor Bunge used to treating DM and search special effects anti-diabetic drug in Chinese Treaditional medicine. The study methods adopted anti-free radical model in vitro and pharmacological study in vivo which is according to free radical theory aboat diabete. These courses is order to select anti-diabetes active ingredient in Potentilla discolor Bunge. Experiment in vitro revealed the activity of different extracts in Potentilla discolor Bunge to eliminate ·OH and inhibit nitration reaction. Experiment in vivo used alloxan induced an