山丹丹鳞茎多糖的提取及含量测定-毕业(学术)论文设计.docVIP

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山丹丹鳞茎多糖的提取及含量测定-毕业(学术)论文设计.doc

PAGE PAGE 9 分类号: R284 单位代码: 109 密 级: 一般 学 号:1090208014031 本科毕业论文(设计) 题 目:山丹丹鳞茎多糖的提取及含量测定 专 业: 生 物 技 术 姓 名: 王 波 指导教师: 王 延 峰 职 称: 副 教 授 答辩日期: 二0一二年六月二日 山丹丹鳞茎多糖的提取及含量测定 HYPERLINK /albums/1490486/1490486/0/0.html \l 0$0d968f231707770cac34de00 \o 查看图片 \t _blank ?? 摘要:山丹丹多糖是山丹丹主要的有效成分,从山丹丹粗多糖中分离纯化出纯化的山丹丹多糖,并对其含量进行测定。用水提醇沉法从植物山丹丹中提取百合粗多糖,再采用Sevage法去除蛋白。除蛋白后的沉淀物依次用无水乙醇、丙酮、无水乙醚各淋洗得到精制百合粗多糖。试验选取固液比、提取时间、提取温度3个因素,在单因素试验的基础上,采用L9 (33)正交试验设计,确定了最佳的提取条件为:提取温度70℃、固液比1:15、提取时间4h。选择此条件提取药用百合粗多糖,提取率为1.263 关键词:山丹丹;多糖;提取;含量测定;正交试验 Bulb of Lilium concolor pellet extraction and content determination of polysaccharide Abstract: Lilium concolor pellet polysaccharide is Lilium concolor pellet main component, from Lilium concolor pellet in the crude polysaccharides purified purified Lilium concolor pellet polysaccharide, and determine the content of. Water extraction and alcohol precipitation from plant extraction of lily Lilium concolor pellet polysaccharide, removing protein by Sevage method. In addition to protein precipitate in ethanol, acetone, ethyl ether the eluent to obtain refined Lily polysaccharide. Test selection ratio of solid to liquid, extraction time, extraction temperature3 factors, based on the single factor experiment, by using L9(33) orthogonal experimental design, the optimum extraction conditions for the extraction temperature 70 degrees1: 15, the ratio of solid to liquid, extraction time4h. Select this condition extracting Lilium polysaccharide, extraction rate of 1.263%. Key words: Lilium concolor pellet; polysaccharide; extraction; content determination; orthogonal test 山丹丹是百合科(Liliaccae)百合属(Lilium)的物种,是多年生草本植物,山丹丹学名斑百合,别名红百合,生活中经常被称为“山丹丹花”,百合科,多年生草本。地下具卵球形鳞茎,有少数白色肉质鳞片。茎直立,高30─60厘米,无毛。叶线状至线状披针形,边缘和背面脉上有乳突。无珠芽。花一至数朵,生于茎端和叶腋内,直立,夏初开花;花被片六枚,两轮,披针形或长椭圆形;朱红色或

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