复方甘草次酸霜对小鼠迟发型超敏反应的防治作用及机制研究-药理学专业论文.docx

万方数据 万方数据 目 录 中文摘要 ·············································································1 英文摘要 ·············································································3 英文缩写 ·············································································5 研究论文 复方甘草次酸霜对小鼠迟发型超敏反应的防治作用及机制研究 前言 ·············································································6 材料与方法 ····································································9 结果 ·············································································19 附图 ·············································································22 附表 ·············································································41 讨论 ·············································································49 结论 ·············································································55 参考文献 ·······································································56 综述 中药单体对迟发型超敏反应免疫抑制作用的研究进展 ·············61 致谢 ···················································································66 个人简历 ·············································································67 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 复方甘草次酸霜对小鼠迟发型超敏反应的防治作用及机制研究 摘 要 目的：本文研究中药制剂复方甘草次酸霜对小鼠迟发型超敏反应 （Delayed type hypersensitivity，DTH）的治疗作用，明确其治疗效果和毒 副作用，并初步探讨其作用机制。 方法： 1 复方甘草次酸霜对脾脏细胞的毒性作用 MTT 法检测甘草次酸体外对小鼠脾脏细胞的毒性，检测其对正常细 胞的杀伤作用，明确其给药剂量下是否对机体具有毒性作用。 2 复方甘草次酸霜对淋巴细胞增殖的作用 MTT 法测定复方甘草次酸霜在体外对刀豆蛋白 A（ConA）及脂多糖 （LPS）诱导的小鼠 T 淋巴细胞和 B 淋巴细胞的增殖抑制作用，以检测甘 草次酸对于淋巴细胞增殖具有抑制作用，并明确其抑制作用的选择性。。 3 复方甘草次酸霜对小鼠迟发型超敏反应的作用 经皮涂抹给药观察不同剂量的复方甘草次酸霜（0.5%、1%、2%）对 小鼠迟发型超敏反应作用后的体重变化、耳肿胀度、耳肿胀重量差、脾脏 指数及 HE 染色检测耳组织病理学变化，通过分析以上指标确定甘草次 酸霜对小鼠 DTH 的治疗作用。 4 复方甘草次酸霜对炎症因子产生的影响 ELISA 法检测不同剂量复方甘草次酸霜对小鼠迟发型超敏反应作用 后炎症因子 IL-1β 和 TNF-α 在小鼠血清内的含量，分析其对体内炎症反应 的作用机制。 5 复方甘草次酸霜对炎症因子基因表达的影响 RT-PCR 法检测不同剂量复方甘草次酸霜对小鼠变应性接触性皮炎 作用后耳组织中炎症相关因子 IFN-γ、IL-6、IL-17a 和 ROR-γt mRNA 表 达，在基因层面上继续研究复方甘草次酸霜的作用机制和作用通路。 结果： MTT 法检测发现复方甘草次酸霜在体外对小鼠脾淋巴细胞无毒性，