富氢水和依达拉奉后处理对乳鼠离体脑片谷氨酸损伤的保护作用以及对NF-κB信号通路的影响-麻醉学专业论文.docx

万方数据 万方数据 结论：离体脑片谷氨酸损伤后，富氢水和依达拉奉后处理通过抑制 NF-κB 和 TNF-α 表达，减少炎症相关因子的表达和释放，减弱氧化应激，抑制炎症反 应，从而对神经元的谷氨酸损伤发挥保护作用。 关键词：氢 脑片 依达拉奉 谷氨酸 后处理 NF-κB III The effect of saturated hydrogen saline and edaravone postconditioning after glutamate injury in rat’s brain Major: Anesthesiology Postgraduate: QI Hai-yan Supervisor: WANG Di-fen [Abstract] Objective: To observe the postconditioning effects of hydrogen-rich saline and edaravone on neonatal rat brain slices in vitro after glutamate injury. Methods: SD neonatal rat cortical brain slices were cultured in vitro for the sixth day, and then divided randomly into 9 groups: group C, group GA; group GA+ED; group GA+H16; group GA+H32; group GA+H64; group GA+H16+ED; group GA+H32+ED; group GA+H64+ED. Each brain slices group was stained by HE after treatment and the TNF-α/NF-κB immunohistochemistry-positive cells were counted, and the number of Nissl staining of normal cells were counted as well. Results: NF-κB and TNF-α immunohistochemical positive cells were significantly increased in glutamate injury group compared with the control group, while the hydrogen-rich liquid and drug treatment groups decreased significantly with the normal number of neurons increased obviously; the hydrogen-rich liquid protection effect was probably correlated with the concentration, and the concentration of 64μmol/L hydrogen and 200μmol/L edaravone provided the best protection. Conclusion: The hydrogen-rich liquid and edaravone could suppress inflammation-related factors such as NF-κB and TNF-α in rat’s brain tissue, and had a protective effect for glutamate neuronal damage. [Key words] Hydrogen; Brain slice; Postconditioning; Glutamate; Edaravone; NF-κB IV 第二部分  富氢水和依达拉奉后处理对乳鼠离体脑片 谷氨酸损伤后 NF-κB 信号通路的影响 专业：麻醉学 研究生：亓海燕 导师：王迪芬 【摘要】目的：通过富氢水和依达拉奉后处理对乳鼠离体脑片谷氨酸损伤保 护作用的研究，进一步探讨两者对脑缺血-再灌注损伤后 NF-κB 信号通路的影响。 方法：本实验通过乳鼠离体脑片谷氨酸损伤建立脑缺血-再灌注损伤模型， 研究富氢水和依达拉奉后处理对乳鼠离体脑片缺血-再灌注损伤的保护作用并进 一步探讨其对 NF-κB 信号通路的影响。体外培养 SD 乳鼠皮质脑片至第六天，随 机分为对照组(Control