粉防己碱对大鼠角膜新生血管作用的实验研究-眼科学专业论文.docxVIP

华中科技大学硕士 学位论 华 中 科 技 大 学 硕 士 学 位 论 文 2 2 Experimental study of Tetrandrine on corneal neovascularization in rats Abstract Objective This study was to investigate the effects of tetrandrine on inflammatory reaction and corneal neovascularization(CNV) following corneal alkali burn，and to explore the expression of the platelet-derived growth factor C(PDGF-C) in CNV. Methods CNV mode was induced by alkali burning in both eyes of 40 rats. right eyes of them were treated by 4×10-5mol/L Tet drop and left eyes were created by 0.9% saline solution for four times daily. The area of CNV was calculated on day 1、 4、7、14、21 later using slit-lamp. Then 11 rats were killed randomly, leukocytes in corneal were quantified by histological study, the expression of PDGF-C were detected by immunochemistry and RT-PCR. Results The area of CNV in Tet group was smaller than that of saline solution group in 4、14、21days (P0.05). Compared with saline solution group, the number of leukocytes infiltration in Tet group was less significantly. Immunohistochemistry and RT-PCR showed the expression of PDEF-C was up-regulated in both Tet group and saline solution group, but was slightly less in the Tet group. Conclusion Corneal injury by 1mol/L NaOH for 30s is a successful way to induce CNV. Tet can partially inhibit the growth of corneal new blood vessel after alkali burning through decreasing leukocytes infiltration and the expression of PDGF-C. Key words Tetrandrine; alkali burn; corneal neovascularization; leukocyte infiltration; platelet-derived growth factor C 独创性声明 本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成 果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或 集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文 中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。 学位论文作者签名： 日期： 年 月 日 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有 权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和 借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据 库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。 本论文属于 保密□ ，在 年解密后适用本授权书。 不保密□。 （请在以上方框内打“√” ） 学位论文作者签名： 指导教师签名： 日期： 年 月 日 日期： 年 月 日 华中科技大学硕士 学位论 华 中 科