低温等离子消融对兔下鼻甲黏膜结构影响的实验观察-耳鼻咽喉科学专业论文.docx

PAGE PAGE 10 目 录 中文摘要??????????????????????2 英文摘要??????????????????????3 前言????????????????????????4 实验材料与方法???????????????????5 结果????????????????????????7 讨论????????????????????????13 结论????????????????????????23 参考文献??????????????????????24 致谢????????????????????????28 综述????????????????????????29 低温等离子消融对兔下鼻甲黏膜结构影响的实验观察 中文摘要 目的： 观察低温等离子消融对兔下鼻甲黏膜的影响，为临床上此手术方式提供实验室依 据。 方法：24 只新西兰白兔，随机取 4 只,其中 2 只取下鼻甲做正常对照，另外 2 只 作为寻找实验合适的低温等离子功率及时间，剩余 20 只随机分为 5 组，每组 4 只， 双侧下鼻甲注射少量生理盐水，左侧行低温等离子消融，右侧做试验对照。在术 后的 1、5、10、15、20 天分别取双侧下鼻甲黏膜标本进行光镜和电镜观察，观察 组织形态学的变化。结果：手术 1 天后下鼻甲外观上出现肿胀及伪膜，5 天肿胀 开始减轻，10 天肿胀消退、伪膜消失，20 天下鼻甲形态恢复正常。光镜和扫描电 镜观察下鼻甲黏膜的结果一致，等离子消融组 1、5 天组下鼻甲黏膜出现上皮细胞 和纤毛的破坏，第 10 天时上皮细胞及纤毛开始修复，至第 20 天时已经完全恢复， 盐水对照组未见黏膜及纤毛的损伤。 结论：下鼻甲低温等离子消融术对下鼻甲黏膜及纤毛有影响。本实验下鼻甲低温 等离子消融术对下鼻甲黏膜及纤毛造成的损伤是可逆的，20 天左右形态结构基本 恢复。选择合适消融的功率及时间行下鼻甲低温等离子消融术十分重要，下鼻甲 低温等离子消融术是一种安全的手术方式。 关键词：下鼻甲，低温等离子射频，鼻炎，黏膜，纤毛 Experimental observation on the impact of low-temperature plasma ablation on the tissue structure of inferior turbinate mucosa in rabbit Abstract Objective:To study the changes of inferior turbinate mucosa after low-temperature plasma ablation in rabbit and to provide laboratory evidence for this clinical surgical approach. Methods: Four rabbits were selected from Twenty-four New Zealand rabbits randomly,take 2 rabbits as normal controls and another 2 rabbits to be used as find the appropriate power and time,other 20 rabbits were divided into 5 groups randomly,4 rabbits in each groups, bilateral inferior turbinate were injected some volume of normal saline，to operate on the left inferior turbinate ,another side as control. Inferior turbinate specimens were respectively taken on the 1,5,10,15,20 days after the operation , the specimens were observed under optical microscope and scanning electron microscope ,and then Compared the histological changes. Results: Swelling and pseudomembranous appeard in the inferior turbinate on the 1st day after operation, it began to reduce swelling on the 5th day, pseudomembra