蛋白磷酸酶抑制剂OA对心房颤动患者心房肌SK2通道功能的调节-生理学专业论文.docxVIP

PAGE PAGE 10 组, 对 SR 组抑制率为 12.51％±4.02 (n=6),对 AF 组的电流抑制率为 32.88% ±9.20 (n=4，P0.05)。结论: AF 组 SK2 电流大于 SR 组；PP2A 参与对 SK2 通道的调控，抑制 PP2A 可使 SK2 电流密度下降，并且对 Apamin 敏感性 降低；AF 时，SK2 通道对钙离子的敏感性增强；SR 组可被 PP2A 影响的 电流成份小于 AF 组。 关键词：心房颤动；小电导钙激活钾通道；蛋白磷酸酶抑制剂；膜片钳技 术 Role of protein phosphatase inhibitoroa on SK2 channels in Right Atrial Myocardium of Patients With Atrial Fibrillation Abstract：Objective：Atrial fibrillation (AF) is the most frequent sustained arrhythmia in the clinical practice, occurring in 1–2% of the general population. Atrial fibrillation is an important risk factor for heart failure and other adverse cardiac events. Atrial electrical remodeling and intracellular calcium regulation abnormalities play an important role in the incidence of atrial fibrillation and maintenance. SK2 is the recent discovery of an important class of calcium-dependent potassium channels. The Ca2-activated K current is expressed differentially in atria compared with ventricles. Because of the marked differential expression of SK2 channels in the heart, specific ligands for Ca2+-activated K+ currents may offer a unique therapeutic opportunity to modify atrial cells without interfering with ventricular myocytes. From another perspective, atrial dysfunction (such as atrial fibrillation) may also indicate the change of the SK2 channel. Kinase and protein phosphatase enzymes in myocardial cells will be abnormal in atrial fibrillation. This becomes of particular importance in the Atrial fibrillation, where abnormalities in the activity of enzymes in the kinase and phosphatase families disturb this fine equilibrium of phosphorylation. SK2 channels are multiprotein complexes and, in addition to the pore-forming subunits and CaM, have constitutively bound protein kinase CK2 and protein phosphatase 2A (PP2A). Abnormal protein phosphatase enzymes likely will have an impact to SK2 channel function. Changes of SK2 channels in atrial fibrillation and protein phosphatase on the SK2 channels r