低分子柑橘果胶联合奥沙利铂对结肠癌生长及肝转移抑制作用的实验分析-外科学专业论文.docx

广州医学院硕士学位论文低分子柑橘果胶联合奥沙利铂对结直肠癌及肝转移抑制作用的实验研究 广州医学院硕士学位论文 低分子柑橘果胶联合奥沙利铂对结直肠癌及肝转移抑制作用的实验研究 PAGE PAGE 4 at a dose of 5mg/kg twice weekly. Mice in Control and LCP group were injected equivalent 5%GS instead of medication. All mice were sacrificed on day 28 after administration. The size of the spleen implanted tumor were measured, and the number of liver metastatic nodules were counted or the diameter of nodules were measured in each group mice. The spleen implanted tumor tissues and liver were resected and collected for further research. The expression of galectin-3 in spleen implanted tumor tissues was detected by immunohistochemistry. Results: The colon cancer and its liver metastasis nude model was established successfully. The percentage of spleen implanted tumor in Control, LCP, Oxaliplatin and Combination group was 93.3%, 93.3%, 92.9% and 93.3% respectively, and shown no significantly differences (all P0.05). The size of spleen implanted tumor in LCP, Oxaliplatin and Combination group were significantly smaller than which in Control group ( P0.05). The size of spleen implanted tumor in Combination group were markedly smaller than which in LCP and Oxaliplatin group (P 0.05). The percentage of liver metastasis in Control, Oxaliplatin, LCP and Combination group was 93.3%, 85.7%, 73.3% and 46.7% respectively. The number of liver metastatic nodules were significantly fewer or the diameter of nodules was markedly smaller in LCP and Combination group compared with which in Control group (P0.05). Compared with LCP group, the number of liver metastatic nodules were significantly fewer or the diameter of nodules were markedly smaller in Combination group (P0.05). The expression of Galectin-3 in spleen implanted tumor tissues in each group were shown no significantly differences (all P=0.859). Conclusion： LCP can inhibit the growth and liver metastasis of colon cancer. LCP combining with Oxaliplatin markedly inhibited the growth and liver metastasis of colon cancer, and th