肺癌患者恶性胸水中的EGFR基因突变检测及与靶向药物的治疗疗效的关系呼吸内科学专业论文.docxVIP

PAGE PAGE IV 肺癌患者恶性胸水中的 EGFR 基因突变检测及与靶向药物的治疗疗效的关系 英文摘要 Detection of EGFR gene mutations in the pleural effusion of lung cancer patients and the relationship with the therapeutic efficacy of targeted drugs Abstract Background: Lung cancer is a serious threat to human health, whose morbidity and mortality is the highest in malignant tumor, and non-small-cell lung cancer takes a proportion of 75 percentage to 80 percentage. Though obvious improvement has been achieved in diagnostic of lung cancer, most of lung cancer patients were diagnostic as advanced stage, have lost the best time operation, only can receive the chemical drug and radiation therapy. The side effect of chemical drug and radiation therapy is serious, some patients can not tolerate it. Recently, the targeted drugs have provided a new hope for lung cancer patients. Targeted drugs as a kind of low molecular compounds taking convenient and having small side effects, but there are individual differences in different patient. A lot of studies have showed a link between somatic mutation of the Exon 18 to 21 in EGFR gene and the responses to targeted drugs. Further study found that almost 90 percentage of EGFR mutations were located in exon 19 and exon 21 of EGFR gene. Now, most EGFR gene mutations were detected from tissue samples, but sometimes they are difficult to obtain tumor samples, especially in patients with inoperable non-small-cell lung cancer, so it is necessary to find some other samples that easily get to detect EGFR gene mutation. Objective: To explore the mutation in exon 19 and exon 21 of EGFR gene in pleural effusion of non-small-cell lung cancer, and evaluated the correlation between the EGFR gene mutation and the therapeutic response to targeted drugs. Methods: Collected 45 cases malignant effusion specimens of non-small-cell lung cancer patients, including 34 cases from the patients who first receive treatment and 11 cases of progress after treatment. All of the pleural effusion fluid w