低分割X线照射同期联合奥沙利铂对大鼠肝脏的损伤性研究-肿瘤学专业论文.docx

万方数据 万方数据 Damage of Wistar rats liver after using Hypo-fractionated radiation and oxaliplation Abstract Objective: The purpose of our study is to clarify whether Hypo-fractionated radiation therapy combined with oxaliplatinon can aggravate liver damage, in order to provide a reference for our clinical application Methods: 80 Wistar rats were randomly divided into four groups: the control group (group A), the chemo-treatment group (group B), the radiation treatment group (group C), and the concurrent chemoradiotherapy group (group D). The liver tissue was collected for histological evaluation at the 1st, 2nd, 4th, 6th and 8th week after irradiation by HE staining. Meanwhile the expression of Bcl-2 and Bax was detected by immunohistochemical technique. Results: Histological examination showed swollen hepatocellular cells in the experimental group. Also, the degeneration and necrosis of liver tissue can be seen. Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) between the groups were significantly different at the same time, (F=85.869,214.663 P0.001). Expressions of Bcl-2 and Bax were significantly different between each time point (F=6.047,43.344 P0.05), and Bax were significantly different between each group (F=8.122 P0.05),but Bcl-2 had no different between each group at the same time(F=0.808 P0.05) Conclusion: Radio-chemotherapy may aggravate liver injury and it must be carefully used clinically. The overexpression of bcl-2 genes and the reduced expression of Bax genes may explain the results. Postgraduate student: ChenMingxuan(oncology) Directed by Prof. An Yongheng Key words: Radiation effects ; Concurrent chemoradiotherapy; Rat liver；Bcl-2；Bax 目 录 引言………………………………………………………………………………………1 第 1 章 实验材料…………………………………………………………………………2 1.1 实验动物……………………………………………………………………………2 1.2 主要药品及试剂……………………………………………………………………2 1.3 主要仪器与设备……………………………………………………………………2 第 2 章 实验方法与步骤…………………………………………………………………3 2.1 动物分组……………………………………………………………………………3 2.2 模型的建立…………………………………………………