紫衫醇对小鼠不同组织巨噬细胞功能的影响.PDFVIP

中国农学通报 2013,29(32):49-53 Chinese Agricultural Science Bulletin 紫衫醇对小鼠不同组织巨噬细胞功能的影响 陈舒楠，官佳懿，谷崇高，芦 山，沈 红 （北京农学院动物科学技术学院，北京102206） 摘 要：为了探讨紫杉醇(Paclitaxel ，PA)对小鼠不同组织巨噬细胞增殖和功能的影响。无菌操作制备小 鼠腹腔、骨髓和脾脏巨噬细胞，在体外细胞培养体系中分别加入不同浓度40 、80、160 ng/mL 的PA 和脂 多糖(LPS)共培养，采用MTT 法检测细胞增殖，ELISA 和Griess 法分别检测细胞分泌TNF-α和NO 量，瑞 氏- 吉姆萨染色法测定腹腔巨噬细胞吞噬能力。结果表明：PA 促进腹腔巨噬细胞和LPS 诱导的腹腔和 骨髓巨噬细胞增殖，但抑制脾脏和骨髓巨噬细胞以及LPS 诱导的脾脏巨噬细胞增殖；PA 抑制不同组织 巨噬细胞分泌TNF-α，但促进腹腔和脾脏巨噬细胞分泌NO ；PA 提高了腹腔巨噬细胞吞噬鸡红细胞的能 力。PA 对小鼠不同组织巨噬细胞的免疫功能均有影响，这为进一步阐明PA 的免疫调节机理提供了新 的理论依据。 关键词：紫杉醇；小鼠；巨噬细胞；免疫功能 中图分类号：S852.16+ 1 文献标志码：A 论文编号：2013-1437 Effects of Paclitaxel on the Functions of Different Tissue Macrophages from Mice Chen Shunan, Guan Jiayi, Gu Chonggao, Lu Shan, Shen Hong (CollegeofAnimalScienceandTechnology,BeijingUniversityofAgriculture, Beijing 102206) Abstract: To investigate the effects of Paclitaxel(PA) on proliferation and function of different tissue macrophages separated from mice. Peritoneal, bone marrow and spleen macrophages were prepared by aseptic technique and in vitro cultured in media containing different concentration of 40, 80 , 160 ng/mL PA alone or respectively together with lipopolysaccharide (LPS). Cell proliferation was detected by MTT, TNF-α and NO level were assayed by ELISA and Griess methods respectively. Phagocytic ability of peritoneal macrophages was analyzed by Wright-Giemsa staining method. The results showed that PA could significantly promote peritoneal macrophage and LPS-stimulated peritoneal and bone marrow macrophages proliferation, but inhibit spleen and bone marrow macrophages and LPS-stimulated spleen macrophages. TNF-α secretion of different tissue macrophages was inhibited by PA, bu