A mutant CHS3 protein with TIR-NB-LRR-LIM domains modulates growth, cell death and freezing tolerance in a temperature- dependent manner in Arabidopsis.英文版本.pdf

The Plant Journal (2010) 63, 283–296 doi: 10.1111/j.1365-313X.2010.04241.x A mutant CHS3 protein with TIR-NB-LRR-LIM domains modulates growth, cell death and freezing tolerance in a temperature-dependent manner in Arabidopsis 1 1 1 1 1 1,2,* Haibian Yang , Yiting Shi , Jingyan Liu , Lin Guo , Xiaoyan Zhang and Shuhua Yang 1 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, and 2National Plant Gene Research Center, Beijing 100193, China Received 9 March 2010; revised 1 April 2010; accepted 15 April 2010; published online 26 May 2010. * For correspondence (fax: +86 10 e-mail yangshuhua@). SUMMARY Low temperature is one of environmental factors that restrict plant growth homeostasis and plant–pathogen interactions. Recent studies suggest a link between temperature responses and defense responses; however, the underlying molecular mechanisms remain unclear. In this study, the chilling sensitive 3 (chs3-1) mutant in Arabidopsis was characterized. chs3-1 plants showed arrested growth and chlorosis when grown at 16C or when shifted from 22 to 4C. chs3-1 plants also exhibited constitutively activated defense responses at 16C, which were alleviated at a higher temperature (22C). Map-based cloning of CHS3 revealed that it encodes an unconventional disease resistance (R) protein belonging to the TIR-NB-LRR class with a zinc-binding LIM domain (Lin-11, Isl-1 and Mec-3 domains) at the carboxyl terminus. The chs3-1 mutation in the conserved LIM- containing domain led to the constitutive activation of the TIR-NB-LRR domain. Consistently, the growth and defense phenotypes of chs3-1 plants were completely suppr