补心软脉颗粒提取纯化工艺的研究.docVIP

基金项目：江苏省科技支撑计划-社会发展项目（BE2011816 基金项目：江苏省科技支撑计划-社会发展项目（BE2011816） 作者简介 范玲（1986-）女 南京中医药大学10级药剂学硕士研究生，Tel Email：fll861031 @126.com *通讯作者：严冬，硕士生导师，主要从事中医临床及中药新药开发研究。Tel：025E-mail：y12d12@163.com 范玲1，李爽 1，马兴苗 1，钱玉良2，刘志辉 2，严冬 1* （1 南京中医药大学，南京210046；2江苏省中医院，南京210036） 摘要: 目的 优选补心软脉颗粒提取纯化工艺。方法 采用正交试验法，以红景天中红景天苷的转移率和浸膏得率作为考察指标进行复方中红景天等五味饮片的水提取工艺的研究，并进行优选和验证。采用单因素试验法，以银杏叶中总黄酮醇苷的转移率作为考察指标进行银杏叶饮片的醇提工艺研究，并进行优选和验证。采用单因素试验法，以红景天中红景天苷的转移率和除杂率作为考察指标进行水提液醇沉工艺的研究，并进行优选和验证。结果：优选的水提工艺为：红景天等五味饮片按处方比例加8倍量的水，提取3次，每次0.5 h；优选的醇提工艺为：银杏叶饮片加10倍量70%的醇，提取2次，每次1h。优选的醇沉工艺为：水提液浓缩至相对密度1.10~1.15，缓缓加入95%乙醇至含醇量70%，边加边搅拌，冷处静置24h。结论 该工艺简便易行。 关键词: 补心软脉颗粒；正交试验；单因素试验；红景天；银杏叶 Study on Process of Extraction of Buxinruanmai Granules FAN Ling 1, LIU Zhi-hui2* (1.Nanjing University of Chinese Medicine, Nanjing 210046; 2.hospital of TCM of Jiangsu province, Nanjing, ABSTRACT: Aim To optimize the technique of extraction of Buxinruanmai Granules. Methods The orthogonal design and Singer factor test was respectively used to optimize and validate water-extracting process with the indices of the transferring rate of salidroside from Rhodioda crenulata and the rate of dried extract and ethanol-precipiting process with the indices of the transferring rate of salidroside from Rhodioda crenulata and imputity rate. Singer factor test was used to optimize and validate ethanol- extracting process with the indices of the transferring rate of total flavonol glycosides .. Results The optimal conditions of water extraction was to decocte 3 times with 8 times water, 0.5 hour for each time and of ethanol extraction was to decocte 2 times with 12 times 70% ethanol, 1 hour for each time .The most optimal alcohol precipitation conditions were as follows: concentrate water extract until its density is 1.10～1.15, add alcohol until concentration of ethanol becomes 70%，precipitated 24 h. Conclusion The technology was simple. KEY WORDS: Buxinruanmai Granules; Orthogonal design; Singer factor test; Rhodioda crenu