壳聚糖固定化绿豆乳糖酶的特性及应用的初步研究-食品科学.PDF

※生物工程 食品科学 2009, Vol. 30, No. 03 227 1 2 1 1 1 1 陈芳艳 ，王林川 ，李文楚 ，王叶元 ，卓孝磊 ，李 洁 (1.华南农业大学动物科学学院，广东广州 510642 ；2.华南农业大学兽医学院，广东广州 510642) ：以壳聚糖凝胶颗粒为载体，采用共价键偶联法固定绿豆乳糖酶，研究壳聚糖固定化绿豆乳糖酶的酶学性 质并对其进行水解牛乳中乳糖的初步应用研究。结果表明：固定化酶的热稳定性在4 ～55 ℃范围内；最适温度 app 60 ℃；最适pH3.5 ；固定化酶的表观米氏常数Km 为0.04% ，溶液酶米氏常数Km 为0.33% ，是溶液酶米氏常数 Km 的0.12 倍；固定化酶的操作半衰期为30d ，较溶液酶长。用固定化酶制备填充床式反应器水解牛奶中乳糖，水 解率可达60% 以上。 壳聚糖固定化乳糖酶；酶学特性；乳糖 Preliminary Study on Characteristics and Application of Chitosan-immobilized Lactase 1 2 1 1 1 1 CHEN Fang-yan ，WANG Lin-chuan ，LI Wen-chu ，WANG Ye-yuan ，ZHUO Xiao-lei ，LI Jie (1.College of Animal Science, South China Agricultural University, Guangzhou 510642, China； 2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China) Abstract：Lactase from mung bean was immobilized onto chitosan surface by using glutaraldehyde as the cross-linking reagent. The results showed that: (1) the immobilized lactase is stable at 4 to 55 ℃; its optimum temperature and pH are 60 ℃ and app 3.5, respectively; (2) its Km [ONPGal] is 0.04%, which is 0.12 times as much as that (0.33%) of free papain; (3) its operation half-life is 30 d, which is longer than that of the free lactase. Furthermore, when this chitosan-immobilized lactase is used in the plug-flow reactor to hydrolyze lactose, the hydrolysis rate is up to more than 60%. Key words ：chitosan immobilizated lactase ；characteristics ；lactose 中图分类号：Q55 文献标识码：A 文章编号：1002-6630(2009)03-0227-04 乳糖酶也被称为β- 半乳糖苷酶，可催化乳糖