壳聚糖复合物对近江牡蛎糖胺聚糖中镉的脱除-食品科学.PDF

46 2014, Vol.35, No.12 食品科学 ※工艺技术 壳聚糖复合物对近江牡蛎糖胺聚糖中镉的脱除 * 郭妍妍，吴红棉 ，衣美艳，范秀萍，陈萌萌，胡雪琼 （广东海洋大学食品科技学院，广东湛江 524088 ） 摘  要：利用凹土-壳聚糖复合物对近江牡蛎糖胺聚糖中的镉进行脱除。考察酶解液的pH值、振荡时间、复合物添 加量和振荡速率对脱镉率和牡蛎糖胺聚糖回收率的影响，并以脱镉率和牡蛎糖胺聚糖回收率为指标，通过响应面法 优化脱镉条件。结果表明：壳聚糖复合物脱除牡蛎中镉的最优条件是：pH 7.2 、振荡时间30 min、添加量6.4 mg/mL、 振荡速率170 r/min，此时镉脱除率为73.7% ，糖胺聚糖回收率为44.1% 。表明凹土-壳聚糖复合物能有效地脱除近江 牡蛎糖胺聚糖中的镉，并且对糖胺聚糖的回收率影响不大。 关键词：凹土；壳聚糖；近江牡蛎；糖胺聚糖；镉 Chitosan Composites for Removal of Cadmium from Glycosaminoglycans of Ostrea rivularis Gould * GUO Yan-yan, WU Hong-mian , YI Mei-yan, FAN Xiu-ping, CHEN Meng-meng, HU Xue-qiong (College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China) Abstract: Attapulgite-chitosan complex was prepared for use in removing cadmium from glycosaminoglycan-rich enzymatic hydrolysates of the entire viscera of Ostrea rivularis Gould. The influence of hydrolysate pH, shaking speed and time, and complex-to-hydrolysate ratio on cadmium removal rates and glycosaminoglycan recovery was explored. By using response surface methodology, the optimized experimental conditions were obtained as hydrolysate pH 7.2, addition of the complex in a proportion of 6.4 mg/mL, and shaking at a speed of 170 r/min for 30 min. The removal rate of cadmium under these conditions was 73.7%, and the recovery of glycosaminoglycan was 44.1%. This study indicates that attapulgite-chitosan complex can effectively remove cadmium from glycosaminoglycan from Crassostrea rivularis, with no significant impact on the recovery of glycosaminoglycan. Key words: attapulgite; chitosan; Crassostrea rivularis Gould; glycosaminoglycan; cadmium 中图分类号：TS254.7 文献标志码：A 文章编号：10