和厚朴酚作用卵巢癌细胞株的蛋白质组学研究-妇产科学(妇产肿瘤)专业论文.docxVIP

和厚朴酚作用卵巢癌细胞株的蛋白质组学研究 Proteomics profde changes in honokiol—treated human ovarian cancer eeHs ● Postgraduate Bian Ce Adviser Prof．Zhao Xia Abstract OBJECTIVES：To compare the alterations in proteomes between honokiobtreated and-untreated human ovarian Callcer SKOV3 cells，and to investigate the mechanism ofantitumor effect ofhonokiol on SKOV3 cells． METHODS：SKOV3 cells were exposed to honokiol(15 pg／mL)for 6 h． ● Then,the cells W@le collected and solubilized and global proteins wore extracted by lysis buffer；two-dimensional elec订ophoresis was conducted with the IPG readystrips勰carriers；the gels WCTe stained with Coomassie blue and alterations between gels were compared by PDQuest．Eventually,8 spots with significant differences were selected and excised and the proteins were identified by ESI-Q—TOF MS analysis． RESULTS：Exposure of SKOV3 cells to honokiol could notably inerease expressions of some proteins，such as Heterogeneous nuclear fibonucleoproteins，Carbonyl reductase，Calcium binding protein， ● Phosphafidylethanolamine#binding protein,Keratin；while expressions of some other proteins decreased,such as Pyruvate killaSe。LIM and SH3 domain,Annexia I Lipocort． 2  和厚朴酚作用卵巢癌细胞株的蛋白质组学研究 CONCLUSION：Exposure of SKOV3 coils to hono／ciol coald／nduce expression changes of some proteins,which wate involved in cellular RNA metabolism，cell differentiation,cell transformation and cell migration,ct a1． ◆ KEYWORDS：proteomics；honokiol；ovarian caⅡcec 2-1)electrophoresis； lllas$spectrometry ●  和厚朴酚作用卵巢癌细胞株的蛋白质组学研究 前 言 卵巢癌(ovari孤cancer)是妇科常见的恶性肿瘤，在女性生殖道肿 瘤中占第三位，次于宫颈癌和宫体癌，而其死亡率居妇科恶性肿瘤首位 ◆ 【11。在大于50岁的人群中卵巢癌的发病率为40／10万人，80％的患者在诊 断时已经处于Ⅲ、Ⅳ期，5年生存率仅为31％[23。卵巢肿瘤发病隐匿，早 期诊断困难。确诊时70％已属临床晚期。先期的手术治疗和化疗对初治患 者缓解率很高，但是多数卵巢癌患者仍会复发。原因是化疗过程中卵巢 癌细胞对化疗药物产生了耐药性，导致了患者预后不良。故在使用传统化 疗药物的同时应积极寻找新的化疗药物作为候补使用。 厚朴是一种重要的传统中药材，是木兰科植物厚朴或凹叶厚朴的干 燥干皮、根皮和枝皮。性温，味苦、辛。具有燥湿消痰。下气除满之功 能。通过与其他草药一起熬制，广泛应用于治疗中风、伤寒症、焦虑以 及神经紊乱等病症【3】。早在1930年日本杉井首先从我国厚朴树皮中分离 得到了厚朴酚。并证明日本厚朴中也含有此种成分，但量很低。1973年 藤田先后从日本厚朴与厚朴中分离得到厚朴酚及其异构体和厚朴酚【41。 作为从厚朴树皮中