猪血酶解产物对大鼠缺铁性贫血预防作用的研究.docxVIP

猪血酶解产物对大鼠缺铁性贫血预防作用的研究 食品与发酵科技 FoodandFermentationTechnology 第48卷(第1期)Vo1.48,Ⅳ0.J 猪血酶解产物对大鼠缺铁性贫血预防作用的研究 胡艳丹,王泷,石磊,郑波,陈一资,黄承钰,李鸣 (1.四川大学华西公共卫生学院,N)lI成都610041;2.N)lI农业大学食品学院,四川雅安625000) 摘要:观察猪血酶解产物对生长期雌性大鼠缺铁性贫血的预防效果.为评价SBEP的贫血预防效果提供实验数据. 本研究利用48只Wistar雌性大鼠按初始血红蛋白和体重随机分为低铁对照组.SBEP低,中,高剂量组.每组12只大 鼠.给低铁对照组大鼠喂饲低铁饲料(5.0mg4A/kg),SBEP低,中,高剂量组大鼠喂饲舍低,中,高剂量SBEP(7,14, 28g/kg)的低铁饲料.每4d称1次体重,实验末取尾血,观察各组大鼠体重的变化;用血细胞自动分析仪检测大鼠Hb, Hct,RBC水平;用全自动血生化分析仪检测血清总蛋白,白蛋白,球蛋白的水平.研究发现,三个剂量组大鼠Hb,Het,RBC 水平均较低铁组大鼠显着增高.差异有统计学意义(Plt;0.05);与低铁对照组比较.一定剂量的SBEP可明显增加血清白蛋 白,球蛋白和总蛋白水平:但实验结束后,大鼠体重增长量各组间的差异均无统计学意义.本研究中猪血酶解产物具有预防 大鼠贫血的作用,可改善体内蛋白质水平,为预防缺铁性贫血和蛋白质营养不良提供了可供选择的营养措施. 关键词:SBEP;预防;缺铁性贫血 中图分类号:TQ93TQ925+.2文献标识码:A文章编号:1674—506X(2012)0l一0030—0003 PreventiveEffectofSwineBloodEnzymolysisProductonIron DeficiencyAnemiainRats HUYan—dan,WANGLong,SHILei,ZHENGBo,CHENYi—zi,HUANGCheng-yu,LIMing (J.WestChinaSchoolofPublicHealth,SichuanUniversity,Chengdu610041,China; 2.CollegeofFoodScience,SwhuanAgriculturalUniversity,Ya’on625014,China) Abstract:Thepreventiveeffectofswinebloodenzymolysisproduct(SBEP)ontheirondeficiencyanemiainwistar ratswasstudied.Forty-eightwistargrowingfemaleratswererandomlydividedintoalow—ironcontrolgroup,three groupsaddedwithSBEPatlow,middleandhighlevelsrespectively.Thecontrolratswerefedwithalow—irondiet;the ratsinthelow,middleandhighdosesgroupswerefedwiththelow—ironcontroldietaddedwithSBEPatdifferent doses(7,14,28SBEPg/ks)for32days.Bodyweightwasmeasuredonceevery4days.Tailbloodofallratswascol— lectedfortheanalysisofHb,HctandRBCbyautomaticbloodcellanalyzer;ofserumtotalprotein,serumalbumin andserumglobulinbyautomaticbloodchemistryanalyzerattheendoftheexperiment.Thestudyshowsthatthe levelsofHb,HctandRBC,serumalbumin,serumglobulinandserumtotalproteinweresignificanthigherinthe 3SBEPgroupsthanthoseinlow-ironcontrolgroup(Plt;O.05).However,nosignificantDifferenceoftheincrease. mentofbodyweightwasobservedbetweenanytwogroups.Itcanbeconsideredthattherearepreventiveeffectsof swinebloodenzymolysisproductonbo