高含量麦角甾醇酵母发酵工艺的优化研究-制药工程专业论文.docxVIP

盘l盒壁麦益甾匪苴醛工艺朐优生匕趟豇摘 盘l盒壁麦益甾匪苴醛工艺朐优生匕趟豇 摘 要 本文采用均匀设计试验方法，计算机软件辅助试验设计，在500ml摇瓶试验 中对酵母优选菌种发酵过程的培养基配方和工艺参数进行了优化控制研究，得到 了最佳工艺条件，即培养基配方为：糖蜜4．0％，添加硫酸铵0．15％、尿素0．05％、 磷酸O．20％、硫酸镁0．13％、碳酸钙O．00％： 培养条件为：接种量10％，500ml 摇瓶装量1 00ml，pH6．5、3 l℃22h培养、摇床转速200r／min。采取上述优化工 艺提高了酵母中麦角甾醇含量，酵母中麦角甾醇含量已达细胞干重的3．9％。模 拟生产条件在30L气升式发酵罐中进行放大研究，得到了“以定量的碳源作底物， 前期一次性加入亚适量的氮源，控制酵母不同生长阶段的发酵温度和pH值，对 数生长期补糖一次(总糖量不变)，达到前期大量增殖细胞以提高生物量，中后 期适当提高温度和pH值促使细胞大量积累麦角甾醇的分段控制”新工艺，酵母 中麦角甾醇含量达到了细胞干重的3．0％～3．2％。将中试研究所得工艺条件放大 到工业化大生产中，在30M3或50M3鼓泡式发酵罐中进行连续35批次的酵母发 酵工业化大生产研究，以获取高含量麦角甾醇酵母，提高麦角甾醇产量，降低维 生素D2的生产成本。工业化大生产中麦角甾醇含量达到了细胞干重的2．3％～ 2．6％，比原来一般大生产水平的1．3％提高了一倍，并接近2000年国际先进大生 产水平(3％)。 关键词： 麦角耐 酵毒磊 工艺蓰衫 流菇、 瘟盒量麦曲甾醒技醛工艺：曲优壬匕碰盐ABSTRACT 瘟盒量麦曲甾醒技醛工艺：曲优壬匕碰盐 ABSTRACT The following PaDer dissertate the optimal conditions of culture medium prescription and technical parameters on yeast fermentation are studied in the 500ml shake-flask．Adopting the advanced Uniformity Design Test assisted by the computer software，we obtain the prime fermentation conditions，which could increase the content of ergosterol in yeast cells．And the ergosterol content could reach to 3．9％ based on eell drv solids in the test．Then we study in the 30I．air-lift fermentor to find the optimal conditions to suit to the industrialized production．We obtain the new technique of subsection control，which is quantitative carbon sources act as the fundus sub—sufficiency ni仃ogen sources are added to medium once in fermentation prophase． the temperature and the pH of medium were controlled in different fermentation stages，and molasses is subjoined to medium once in the logarithm growth phase(but the tota．1 molasses is invariable)．In short，it could multiply abundant yeast cells to increase the biomass in the propbase and urge to accumulate ergoster01 in yeasts improving properly the temperature and the pH in the metaphase and anaphase of fermentation．The ergosterol content could reach to 3．0％to 3．2％in this test．At last we magnify the test in the industrialized produc