水稻雄性不育突变体w122鉴定和初步定位.PDFVIP

第10卷第15 期 Vol.10 No.15 2017 年 8 月 August 2017 水稻雄性不育突变体 w122 的鉴定与初步定位 尚丽娜，刘 梅，刘飞燕，方顺然，郑尧杰，王 楠* （西南大学水稻研究所，重庆 400715 ） 摘要：利用甲基磺酸乙酯（EMS ）诱变获得了一个水稻雄性不育突变体w122. 表型分析结果显示，与野生型 相比，w122 突变体雄蕊形态正常，但颜色呈浅黄色，I2-KI 染色结果显示w122 的花粉育性为半不育。此外， w122 突变体株高相较于野生型显著降低，分蘖数显著减少。遗传分析结果表明，w122 突变体的雄性不育性状 受一对隐性单基因控制，通过基因的初步定位，将w122 突变位点定位在引物FL-1 和W25-38 之间 1.23 M 的 物理距离内。 关键词：作物遗传学；水稻；雄性不育；基因定位 中图分类号：S330 文献标识码：A 文章编号：1674-2850(2017)15-1713-09 Identification and gene initial mapping of rice male sterile mutant w122 SHANG Lina, LIU Mei, LIU Feiyan, FANG Shunran, ZHENG Yaojie, WANG Nan (Rice Research Institute, Southwest University, Chongqing 400715, China) Abstract: In this study, the sterile rice male mutant w122 was identified by EMS. Phenotypic analysis showed that the stamens of w122 mutant were normal, but the color was in pale yellow compared with wild type. I2-KI staining showed that the pollen fertility of w122 was semi-sterile. In addition, the plant height of the w122 mutant was significantly reduced compared with wild type and the number of tillers was significantly reduced. The results of genetic analysis showed that the male sterile traits of w122 mutant were controlled by a pair of recessive single genes. Gene mapping results showed that the genetic loci of w122 mutant were located within 1.23 M of physical distance between primers FL-1 and W25-38. Key words: crop genetics; rice (Oryza sativa L.); male sterility; gene mapping 0 引言 水稻（Oryza sativa L. ）是最重要的粮食作物之一，与其他同科经济作物相比，具有非常直接的经济 价值。此外，与其他作物如玉米、油菜等相比，由于水稻的基因相对较小，仅约373 Mb，全基因组测序 完成，基因序列信息较为明确，转基因技术成熟，易于实现转基因，且水稻的起源与其他禾