昆明小鼠胚胎干细胞分离培养方法.pdfVIP

中国组织工程研究与临床康复彳争，2眷彳穸2，筋2008—05—20出版 Rehabilitative而ssueResearch 器1已是基础医学 JournalofClinical EngineeringMay20,2008V01．12,No．21 昆明小鼠胚胎干细胞的分离培养方法术★ 李卫东，张晓刚，常静，蒋芳萍 Isolationandcultureofroutine stemcellsof embryonic Kunmingspecies LiWei·dong，ZhangXiao-gang，ChangJing，JiangFang·ping Abstract Cko∞nIm of of arecollected BACKGRoUND：Hundredsof stemeelIlinesareestablished thesecelllines from m叫∞embryonic presently．Most Rrst C二aadiology, rate celllineswitll miceis low． 129．C57BL／6JandBALB／Cmice．Success stem A伍km。dH∞Ditalof ofcreatingembryonic Kunmingvery themethod andcultureofmurine stemcellsof toincre．as,ethe MedicaloBJECTⅣE：To ofisolation embryonic Chongqing explore kunmingspecies Universky,Chongqing of of stemcelllinesfrom mouse． efficiencyestablishmentembryonic Kunmingspecies 4000l61China DESIGN．TIMEANDSETTING：necell was atthe of from experimentperformedChongqingKeyLaboratoryNeurologyApri／ Ij W西姗★， 2006toJune2007． StudyingforⅡnIle，s of were 3．5davsand4 from mousecollected． MATERIALS：Blastocystsdays Kunmingspecies deP隅nlyiciaq