基于特异性基因的副溶血性弧菌快速分离鉴定-食品与生物技术学报.PDFVIP

第30 卷第3 期 食 品 与生 物 技 术学 报 Vol. 30 No.3 2011年5 月 Journal of Food Science and Biotechnology M ay 2011 : 2011 基于特异性基因的副溶血性弧菌快速分离鉴定 翁仕强, 赵 勇, 潘迎捷, 卢瑛 (上海海洋大学食品学院, 上海201306) : 副溶血性弧菌(Vibr io p ar ahaem oly ticus ) 是海洋环境中 见的食源性致病菌采用自行 设计的改良初筛方案结合tl h tox R 这2 种特异性基因的2 次筛选开发了一种副溶血性弧菌的快 速分离鉴定方法应用该法从海水海泥及3 种海产品中分离得到了19 株副溶血性弧菌, 再经 16S rDNA 测序对分离鉴定结果的特异性进行了验证实验结果显示基于tl h tox R 这两种特异 性基因分离鉴定所得19 株菌的准确性为100%, 全程检测时间为3 d上述结果显示本研究开发 的基于特异性基因的副溶血性弧菌分离鉴定法是一种高效快速的检测方法,特异性强且耗时短, 在食源性致病菌的风险评估和大规模样本的分析检测领域具有较高的潜在应用价值 : 副溶血性弧菌; 特异性基因;tl h; tox R ; 鉴定 : S 37 : A Rapid Isolation and Identif ication Method Based on Specif ic Gene for Vibrio p araha emoly t icus WENG Shiqiang, ZHAO Yong, PAN Yingjie, LU Ying (College of Food Science and T echnology, Shanghai Ocean University, Shanghai 201306, China) Abstract : Vibr io Parahaemoly ticus is a frequent food- borne pathogen in arine environ ents. A rapid isolation and identification ethod for Vibrio Parahae olyticus was developed in this study by co bining an i proved screening ethod with a second screening based on specific genes ( tl h and t ox R) . By using this ethod, 19 strains of Vi br i o p ar ahaemoly t ic us were isolated fro arine water, oozeand three arineproducts. Also, the 16S rDNA fro theisolated strains was sequenced to validate the specificity. The results obtained by the specific t lh, tox R gene identification ethod were consistent with that fro 16S rDNA sequencing, shown that the veracity of the identification ethod based on specific gene is 100%. Our results appeared that the isolation and identification ethod based on specific genes