低温生淀粉糖化酶菌株RS01分离及其酶学性质-微生物学报.PDF

微生物学通报 JUN 20, 2010, 37(6): 798−802 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 低温生淀粉糖化酶菌株 RS01 分离及其酶学性质 1 1 2 1 1* 孙子羽 迟乃玉 王宇 李兵 张庆芳 (1. 大连大学生物工程学院 辽宁省海洋微生物生物工程技术研究中心 辽宁 大连 116622) (2. 大连工业大学 生物与食品工程学院 辽宁 大连 116034) 摘 要: 自渤海湾海泥中分离得到一株产低温生淀粉糖化酶能力较强的菌株RS01, 经形态学、生 理生化特性及 16S rRNA 分析将其鉴定为气单胞菌属。对该菌株产的低温生淀粉糖化酶酶学性质 进行初步研究, 结果表明其最适酶反应温度为 30°C, 酶的热稳定性比较差, 最适 pH 为 5.4, 经 TLC 鉴定酶解产物中有葡萄糖, 表明该分离菌株具有产低温生淀粉糖化酶的能力。 关键词: 低温生淀粉糖化酶, 气单胞菌, 酶学性质 Characterization of Cold-active Raw Starch-digesting Glucoamylase from Isolated Strain RS01 SUN Zi-Yu1 CHI Nai-Yu1 WANG Yu2 LI Bing1 ZHANG Qing-Fang1* (1. Liaoning Technology of Marine Microbiological Engineering Research Center, Bioengineer College, Dalian University, Dalian , Liaoning 116622, China) (2. School of Bio Food Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China) Abstract: A strain producing cold-active raw starch-digesting glucoamylase was isolated from Bohai Bay’ samples. The strain RS01 was identified as Aeromonas sp. based on the morphological, physio- logical characteristics and 16S rRNA sequence. The foundation on the enzymatic properties of partially purified Cold-active RSGA explained that the maximum activity of the enzyme was exhibited at 30°C and pH 5.4. The enzyme thermal stability was poor. The glucose was identified by the TLC, which proved that th