人尿组织激肽释放酶对兔症状性脑血管痉挛早期扩张脑血管效应的评价.docVIP

人尿组织激肽释放酶对兔症状性脑血管痉挛早期扩张脑血管效应的评价 裴圣林 王均炉 胡章勇 莫云长 周乐平 陈伟建 【摘要】 目的 评价人尿组织激肽释放酶（human urinary Tissue Kallikreins HTK）对兔症状性脑血管痉挛( cerebral vasospasm, CVS) 扩张脑血管效应及神经功能的影响。方法 采用Endo法建立家兔脑血管痉挛模型,将日本大耳兔随机平均分为4组,即生理盐水对照组(枕大池注入生理盐水,A组) 、蛛网膜下腔出血组(B组) 、人尿组织激肽释放酶治疗组(C组)及尼莫地平治疗组(D组)。对每组动物分别行行为学评分、神经功能评分和脑血管造影。结果 B组家兔食量明显降低，神经症状明显；而C、D组食量减少、神经症状都较B组程度轻；A组基本正常。B组兔基底动脉在SAH后4、7天痉挛明显，分别为(1.49 ± 0.33 vs 1.86 ± 0.34，P=0.0060.01)、(1.39 ± 0.27 vs 1.86 ± 0.34, P=0.0010.01)；在SAH后7、14天兔基底动脉痉挛C组较B组改善明显，分别为(1.71 ± 0.24 vs 1.39 ± 0.27, P0.001)、 (2.10 ± 0.45 vs 1.75 ± 0.34, P0.001)；而D组在4、7、14天兔基底动脉痉挛较B组改善不明显。结论 人尿组织激肽释放酶和尼莫地平均能较明显地改善兔症状性脑血管痉挛的神经症状，但前者早期缓解血管痉挛的效果较尼莫地平更好。 【关键词】 症状性脑血管痉挛；蛛网膜下腔出血；人尿组织激肽释放酶；尼莫地平 Effects of human urinary Tissue Kallikreins on vasodilation of basilar artery in rabbits with symptomatic cerebral vasospasm. PEI Sheng-lin, WANG Jun-lu, HU Zhang-yong, ,MO Yun-chang, ZHOU Le-ping, CHEN Wei-jian. Department of Anesthesiology, First Affiliated Hospital of Wen Zhou Medical College , Wenzhou 325000, China 【Abstract】Objective To evaluate the effects of urinary kallidinogenase on SAH in rabbits. Methods　forty rabbits were divided into 4 groups, the control group, SAH group, Nimodipine group and urinary kallidinogenase group. Changes of food intake, neurological deficit and degree of vasospasm of basilar artery were observed after SAH. Results　Food intake decreased and neurological deficitwere seen in SAH group, while which were attenuated in group C and group D. As compared the diameters of basilar