骨髓间充质干细胞移植对慢性脑缺血大鼠认知功能及海马CA_1区EphB2的影响.doc

骨髓间充质干细胞移植对慢性脑缺血大鼠认知功能及海马CA1区EphB2的影响 李晓晓 张博爱 李俊敏 刘宇 于晓云 崔璨 摘要 目的 观察骨髓间充质干细胞移植（mesenchymal stem cells，MSCs）对慢性脑缺血大鼠认知功能及海马CA1区EphB2的影响。 方法 将SD大鼠随机分为假手术组、模型组、实验组（2VO模型+MSCs干预），选8、10、12周三个时间点，采用双侧颈总动脉永久性阻断法（2VO）建立慢性脑缺血模型，通过Morris水迷宫检测各组大鼠的认知功能，同时用免疫组织化学方法和Western blot检测大鼠海马CA1区EphB2的表达。结果 模型组和实验组大鼠与假手术组相比逃避潜伏期明显延长, 同一时间点，实验组逃避潜伏期较模型组明显缩短(P 0. 05) , 实验组海马CA1区EphB2的表达较模型组增多（P 0.05 )。结论 骨髓间充质干细胞移植能明显改善大鼠慢性脑缺血所致认知功能障碍, 其可能是通过升高EphB2的表达而改善学习记忆能力。 关键词：骨髓间充质干细胞（MSCs）； 慢性脑缺血 ；EphB2 ；海马 ；认知功能 中图分类号： R743 Transplantation of mesenchymal stem cells could improve the cognitive function and increase the expression of EphB2 protein in Hippocampus of chronic cerebral ischemia rats LI Xiaoxiao, Zhang Boai, Li Junmin，et al.( The Frist Department of Neurology， the First Affiliated Hospital of Zhengzhou University，Zhengzhou 450052，China) Abstract: Objective To investigate the effect of mesenchymal stem cells transplantation on cognitive impairment and the expression of EphB2 in Hippocampus CA1 pyramidal cells of the chronic cerebral?ischemic rats. Methods Rats were randomly divided into Sham operation group 、 model group(permanent ligation of bilateral common carotid arteries,2VO) and MSCs group .Each group was subdivided into 8，10 and 12 weeks.Cognitive function was evaluated by the Morris water?maze and the expression of EphB2 protein was measured using Western blotting and HYPERLINK app:ds:immunohistochemistry \t _self immunohistochemistry.Results In the Morris water maze test, the escape latency of the model group and MSCs group obviously extended compared to the Sham operation group(P0.05), while the escape latency in the MSCs group shorter than model group(P0.05), the expression of EphB2 in hippocampal CA1 areas in the MSCs group significantly increased than the model group by HYPERLINK app:ds:immunohistochemistry \t _self immunohistochemistry and Western blot test (P0.05).Conclusions MSCs transplantation could improve cognitive function of chronic ce