超高压液相色谱-飞行时间质谱法分析国产油橄榄叶中酚类-食品科学.PDF

※分析检测 食品科学 2011, Vol. 32, No. 18 225 1 1 1 2 2 1 , 王百川 ，付绍平 ，王 丹 ，王龙星 ，肖红斌 ，朱靖博 * (1.大连工业大学食品学院，植物资源化学与应用研究所，辽宁 大连 116034 ； 2. 中国科学院大连化学物理研究所，辽宁 大连 116023) 建立国产油橄榄(Olea europaea L.) 叶中酚类化合物的超高压液相色谱- 飞行时间质谱(ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry ，UPLC/Q-TOF-MS) 定性分析方法。液相色谱条件为 Waters BEH-C18(10.0mm ×2.1mm ，1.7μm)色谱柱，乙腈和甲酸溶液梯度洗脱，流速 0.25mL/min ，紫外检测波长 280nm ；质谱条件为Waters ACQUITY Q-TOF-MS 质谱仪，电喷雾电离接口，负离子模式检测。采用该方法得 到了国产油橄榄叶提取物的紫外检测的色谱图、负离子监测的总离子流图以及一级质谱图，成功鉴定了国产油橄榄 叶中的 15 种酚类化学成分。 超高压液相色谱 - 飞行时间质谱法(UPLC/Q-TOF-MS)；国产油橄榄叶；酚类化合物 Characterization of Phenolic Compounds in Domestic Olive Leaf by Ultra-performance Liquid Chromatography/ Electrospray Ionization Quadrupole Time-of-flight Tandem Mass Spectrometry 1 1 1 2 2 1, WANG Bai-chuan ，FU Shao-ping ，WANG Dan ，WANG Long-xing ，XIAO Hong-bin ，ZHU Jing-bo * (1. Institute of Chemistry and Applications of Plant Resource, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China ；2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China) Abstract ：An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was established to characterize phenolic compounds in domestic olive (Olea europaea L.) leaf. UPLC separation was performed on a Waters BEH-C18 column (10.0 mm ×2.1 mm, 1.7 μm) using a mobile phase consisting of 0.1% formic acid water solution (A) and acetonitrile (B).