超高效液相色谱法测定化妆品中对羟基苯乙酮含量-食品安全质量检测.PDF

第8 卷 第9 期 食品安全质量检测学报 Vol. 8 No. 9 2017 年9 月 Journal of Food Safety and Quality Sep. , 2017 超高效液相色谱法测定化妆品中对羟基苯 乙酮含量 刘玉玲, 潘小红, 殷 帅, 姜成君, 孟庆玉* (湖南省药品检验研究院, 长沙 410001) 摘 要: 目的 建立超高效液相色谱法(ultra performance liquid chromatography, UPLC)测定化妆品中对羟基 苯乙酮含量的分析方法。方法 样品前处理以甲醇为溶剂, 超声30 min, 采用色谱柱Atlantis® d C 18 分离, 以 甲醇-水(25:75, V:V)为流动相, 流速为0.2 mL/min, 检测波长为275 nm, 经UPLC 检测; 同时对提取剂和提取 时间、流动相比例、检测波长等条件进行优化。结果 对羟基苯乙酮浓度在0.4376~21.83 μg/mL 范围内呈良 好的线性关系, 相关系数为0.9996, 膏霜、乳、液等不同剂型样品回收率高, 平均回收率分别为99.89%、99.83% 和 100.24%, RSD 分别为0.75% 、0.65%和0.46% 。方法检出限为1.38 μg/g 。结论 该方法简单、快速、准确, 可适用于化妆品中对羟基苯乙酮含量的测定。 关键词: 对羟基苯乙酮; 超高效液相色谱法; 化妆品 Determination of 4-hydroxyacetophenone in cosmetics by ultra performance liquid chromatography * LIU Yu-Ling, PAN Xiao-Hong, YIN Shuai, JIANG Cheng-Jun, MENG Qing-Yu (Hunan Institute for Drug Control, Changsha 410001, China) ABSTRACT: Objective To establish a method for the determination of 4-hydroxyacetophenone in cosmetics by ultra performance liquid chromatography (UPLC). Methods Samples were extracted with methanol by ultrasonic extraction for 30 min, and separated by an Atlantis® d C 18 column with methanol-water (25:75, V:V) as mobile phase at the flow rate of 0.2 mL/min, and then detected by UPLC under the detection wavelength of 275 nm. Meanwhile, extr