大鼠C6脑胶质瘤生长规律的影像学与病理学观察.docVIP

PAGE PAGE 5 大鼠C6脑胶质瘤生长规律的影像学与病理学观察基金项目： 基金项目：重庆市攻关课题(CSTC, 2007AC5014) Supported by the Foundation for Sci Tech Research Project of Chongqing (2007AC5014) 作者简介：王博(1983－)，女，河南省禹州市人，汉族，硕士研究生，医师，主要从事神经放射学方面的研究。电话E-mail：wangbo0374@yahoo.cn 通讯作者：张伟国，电话：023王博，王毅，陈金华，谭勇，李雪，张伟国 (第三军医大学大坪医院野战外科研究所放射科，重庆 400042) 提要：目的 建立Wistar大鼠C6胶质瘤模型，采用MRI、CT灌注(CT perfusion, CTP)成像观测肿瘤体积的动态变化，探讨大鼠C6脑胶质瘤的生长规律。方法 成年Wistar大鼠40只，采用立体定向仪进行C6细胞脑内接种，建立大鼠C6脑胶质瘤模型。每次随机抽取10只接种鼠分别对应于5~10d、10~15d、15~20d三个时间段行CTP、MRI及病理学检查，观测大鼠C6脑胶质瘤的生长规律，并评价影像学与病理学结果之间的相关性。结果 15~20d时间段CTP、MRI观测结果分别与病理学结果比较均有显著性差异(t＝3.131, P0.01；t＝2.566, P0.05)，但CTP与MRI观测结果差异无统计学意义(P0.05)。5~10d和10~15d时间段三种技术观测结果比较均无统计学意义(P0.05)。Pearson线性相关分析表明CTP、MRI与病理学三者测量结果间均有显著正相关，病理学与CTP、病理学与MRI、CTP与MRI的r＝0.998, 0.998, 1.000(P均0.01)。回归分析表明，肿瘤体积随时间呈指数规律增长(rpatho＝0.990, rCTP＝0.987, rMRI＝0.990, P均0.01)。结论 影像学活体观测的准确性高，更真实反映肿瘤的实际大小，适于对肿瘤动物模型的生长观测和试验性治疗的疗效评价。 关键词：胶质瘤；动物模型；灌注成像；磁共振成像 中图分类号：R73-352 R816.1 Growth pattern of rat C6 brain gliomas: imaging and histopathologic observation WANG Bo, WANG Yi, CHEN Jin-hua, TAN Yong, LI Xue, ZHANG Wei-guo (Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China) Abstract: Objective To establish a Wistar rat model of C6 brain glioma and explore the growth pattern in C6 brain glioma rats by MRI and CT perfusion (CTP) imaging. Methods Rat model of brain glioma was established by implanting C6 cells into 40 adult Wistar rats with stereotactic technique. CTP, MRI and pathologic examination were performed in 10 implanted rats randomly sampled at the time periods of 5-10 d, 10-15 d, 15-20 d?after tumor implantation. The growth pattern of C6 brain glioma in rats was observed and the correlation between imagings and histopathlogic results was evaluated. Results There had significant difference at 15-20 d (t=3.131, P0.01; t=2.566, P0.05) between CTP, MRI and histopathologic results, but no significant difference (P0.0