腺病毒介导的神经生长因子基因治疗大鼠坐骨神经损伤的疗效研究.docVIP

腺病毒介导的神经生长因子基因治疗大鼠坐骨神经损伤的疗效研究 陈渝，王大勇，王祖贵，范大礼，翁政（重庆市第九人民医院，重庆 400700） 摘 要：目的 观察腺病毒介导神经生长因子(Ad-NGF)基因治疗对大鼠坐骨神经损伤修复的疗效。方法 取SD大鼠16只，切断右侧坐骨神经并缝合，随机分为Ad-NGF治疗组、神经生长因子(NGF)局部注射组、空腺病毒载体组和生理盐水(NS)局部注射组。术后第31天行坐骨神经功能指数及神经电生理测定，Western blotting检测神经生长因子蛋白质表达水平。结果 腺病毒介导神经生长因子较其余三组可显著增加局部NGF蛋白质表达水平，改善坐骨神经功能指数和神经传导速度。结论 腺病毒介导神经生长因子基因治疗可有效促进大鼠坐骨神经损伤后修复。 关键词：坐骨神经；神经生长因子；神经损伤；腺病毒 中图法分类号：R68 文献标识码：A Effect of adenovirus expressing nerve growth factor on sciatic nerve injury in rats CHEN Yu, WANG Da-yong, WANG Zu-gui, FAN Da-li, WENG Zheng (Department of Orthopedics, Chongqing Ninth People’s Hospital, Chongqing 400700, China) Abstract: Objective To investigate the effect of adenovirus expressing nerve growth factor (Ad-NGF) on sciatic nerve injury in rats. Methods Sciatic nerve injury model was established by transection of right sciatic nerve in 16 male SD rats, and then randomly divided into four groups: Ad-NGF group, nerve growth factor (NGF) group, adenovirus vector group and normal sodium group. The effect of various treatments on injured sciatic nerve was evaluated by examining sciatic nerve function index (SFI) and protein expression of NGF by Western blotting, performing electrophysiological test on day 31 after operation. Results Ad-NGF increased the protein expression of NGF and improved SFI and nerve conduction velocity significantly, as compared with the other three groups. Conclusion Ad-NGF can effectively promote the repair of sciatic nerve injury in rats. Key words: sciatic nerve; nerve growth factor; nerve injury; adenovirus 周围神经完全损伤后，所属感觉、运动和营养作用丧失，对肢体功能影响很大，若不修复神经，肢体功能不易恢复。神经生长因子（nerve growth factor, NGF）作为NGF家族第一个被发现的成员，大量研究已经证明其具有促进中枢及外周神经系统中神经元的存活、分化、促进损伤神经的轴突再生、防止细胞坏死的功能[1]。但局部神经、肌肉注射等给药方法常导致NGF浓度波动大，影响了NGF的临床疗效。本研究拟采用自行构建携带神经生长因子基因序列的5型腺病毒(adenovirus expressing nerve growth factor, Ad-NGF)局部注射，观察Ad-NGF基因治疗对周围神经损伤的修复作用，探讨基因治疗在周围神经损伤中的应用。 1 材料与方法 作者简介：陈 渝.男，重庆北碚区人，硕士，主治医师，主要从事脊柱及周围神经损伤的研究。电话：（023）607