转录因子Tbx18在小鼠胚胎发育过程中的时空表达.docVIP

转录因子Tbx18在小鼠胚胎发育过程中的时空表达 蒲 荻1, 杜建霖2,李晓群3,翁敏杰4,佘 强 (400010重庆，重庆医科大学附属第二医院心血管内科) [摘要] 目的 检测胚胎期小鼠Tbx18 在mRNA及蛋白水平的表达情况，了解Tbx18在小鼠胚胎发育过程中的时空表达模式。方法 用整体原位杂交技术检测小鼠整胚TBX18 mRNA的表达，用 X-gal染色检测Tbx18-Cre/Rosa26R/LacZ双杂基因谱系示踪模型小鼠胚胎的报告基因LACZ蛋白的表达，用荧光定量PCR检测小鼠胚胎期心脏mRNA的定量表达。结果 发现小鼠胚胎转录因子Tbx18在9.5~10.5 d最早主要在前体心外膜表达，由前体心外膜逐渐迁移定位于心外膜；在11.5~12.5 d小鼠胚胎Tbx18主要定位于心脏、上下肢、体节及上下唇鄂区域；大于12.5 d Tbx18除了上述部位外还定位于肾、输尿管、膀胱等区域。结论 转录因子Tbx18在小鼠胚胎发育过程中的表达具有时空特异性，对小鼠心脏的发育可能起着至关重要的作用；同时在上下肢、体节、唇鄂、肾、输尿管、膀胱的发育中也可能起非常重要的作用。 [关键词] 转录因子Tbx18；整体原位杂交；小鼠；胚胎发育；时空表达 [中图法分类号] 【文献标志码】A Spatiotemporal Expressions of The Transcription Factor Tbx18 in Mouse Embryonic Development Pu Di1，Du Jianlin，Li Xiaoqun，Weng Minjie，She Qiang （(The second affiliated hospital Chongqing medical university cardiovascular branch, Chongqing, 400010 ) [Abstract] objective To detected the mRNA and the protein expression of transcription factor Tbx18 and reveal the spatiotemporal expressions in mouse embryonic development. Method We detect whole embryos Tbx18 mRNA expressions in the process of mouse embryonic development using whole mount in situ hybridization technology， detect the reporter LacZ gene’s protein expression of Tbx18-Cre/Rosa26R/LacZ genetic lineage tracer model mouse embryos using X-gal strain technology, detect the quantity of whole mouse embryonic hearts mRNA using fluorescence quantitative PCR. Results The Tbx18 signals of E9.5d to E10.5d mice embryos mainly expressed in the proepicardium at first and then emigrated to the epicardium gradually. The Tbx18 signals of E11.5d to E12.5d mouse embryos mainly expressed in epicardium, forelimb bud and hindlimb bud, somite, mandibula/maxillar cleft region. Besides the regions above, the Tbx18 signals of older than E12.5d mouse embryos also expressed in the kidney, ureteral and bladder. Conclusion The expression of the transcription factor Tbx18 has spatiotemporal idiosyncrasy in the mouse embryonic development. It may plays a vital role in the mous