锌离子结合相关基因mRNA在耐药性癫痫患者颞叶组织中的表达.docVIP

锌离子结合相关基因mRNA在耐药性癫痫患者颞叶组织中的表达 杨柳1，王学峰2 （1.重庆市急救中心神经内科，400014；2.重庆医科大学附属第一医院神经内科，重庆，400016） 【摘要】 目的 研究耐药性癫痫患者颞叶组织内与锌离子结合相关的基因mRNA的表达，探索其在耐药性癫痫发病机制中的作用。 方法 收集48例耐药性癫痫患者术后的颞叶组织，在基因芯片扫描预期结果的基础上，运用RT-PCR检测与锌离子结合相关的TRAF3，RYBP，CNOT4和NR4A2基因在耐药性癫痫患者颞叶中的表达。结果 较8例对照组，基因芯片扫描显示与锌离子结合相关的4个基因TRAF3，RYBP，CNOT4和NR4A2在耐药性癫痫患者脑组织中的表达明显增加，RT-PCR检测获得相同的结果。结论 与锌离子结合相关的基因TRAF3，RYBP，CNOT4和NR4A2可能参与了耐药性癫痫的形成，并在耐药性癫痫的发病机制中起着一定的作用。 【关键词】耐药性癫痫，锌结合相关基因，基因芯片，RT-PCR Expression of genes related zinc-binding in the temporal lobe of the patients with drug-resistant epilepsy Yang Liu1, Wang Xuefeng2 1. Department of Neurology, Emergency Center of Chongqing, 400062; 2. Department of Neurology, the First Affiliated Hospital, Chongqing Medical University, Chongqing, Abstract Objective To explore the molecular mechanism of drug-resistant epilepsy through the diferential expression of the genes related zinc-binding mRNA in the temporal lobe of the patients with drug-resistant epilepsy. Methods The microarray scanning and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expressions of genes related zinc-binding TRAF3, RYBP, CNOT4 and __________________________________ 作者简介： 杨柳（1979-），硕士，研究方向：难治性癫痫的机制； 通讯作者：王学峰，博士导师，Email：xfyp@163.com 基金项目：国家自然科学基金（No.和重庆市卫生局重点项目资助及两江学者专项经费资助 NR4A2 mRNA in the temporal lobe of 48 patients with drug-resistant epilepsy and 8 normal human as a control. Results The expression of TRAF3, RYBP, CNOT4, NR4A2 mRNA were significantly higher in the patients with drug-resistant epilepsy than that in the control, which were identical with the gene microarray. Conclusion Increased expression of TRAF3, RYBP, CNOT4, NR4A2 related with zinc-binding in the human brain may be associated with human drug-resistant epilepsy, and zinc seems to play a role in the pathogenesis of drug-resistant epilepsy. Key words drug-resistant epilepsy, zinc-binding, microarray scanning,RT-PCR 锌是人体内一种重要的微量元素，参与了细胞膜的稳定、酶和DNA的合成、激素的合成分泌、机体的生长发育等过程。锌在脑的海马中的浓度很高，并