新抗原基因MLAA-34在急性单核细胞白血病患者的表达及意义.docVIP

新抗原基因MLAA-34在急性单核细胞白血病患者的表达及意义? ?[基金项目] 国家自然科学基金面上项目? Supported by the General Program of ?National Natural Science Foundation of China 作者简介：赵建强，男，博士，主治医师，主要研究方向：血液肿瘤的免疫治疗。E-mail： HYPERLINK mailto:zjqzy06@163.com zjqzy06@163.com. 通讯作者：张王刚，电话（029E-mail：xianblood@163.com 赵建强 ，张王刚， 何爱丽， 张文娟， 古流芳 西安交通大学医学院第二附属医院血液内科，陕西西安 710004 摘要 目的 探讨白血病新肿瘤抗原基因MLAA-34在急性单核细胞白血病（AML-M5）初诊患者中的表达水平，分析其与临床特征及预后的关系。方法 采用基于TaqMan探针的实时荧光定量RT-PCR方法检测MLAA-34在AML-M5患者35例、非AML-M5急性髓细胞白血病患者40例、非白血病的血液疾病患者12例骨髓标本以及健康志愿者10例外周血单个核细胞中的表达水平并进行比较。结果 初治AML-M5患者MLAA-34 mRNA表达水平较正常对照组、非AML-M5白血病明显增高，差异有显著性（p＜0.05) ；完全缓解的M5患者MLAA-34 mRNA表达水平较治疗前明显下降（p＜0.05)，复发的M5患者MLAA-34 mRNA表达水平再次增高；对8例复发的AML-M5患者进行MLAA-34基因表达水平动态监测，发现复发前MLAA-34基因表达水平有上升趋势。结论 MLAA-34基因的过表达参与了急性单核细胞白血病的发病，在AML-M5中的表达具有较高的特异性，动态监测其表达水平在AML-M5的评估疗效、判断预后及复发预测方面具有一定的临床意义。 关键词 MLAA-34；急性单核细胞白血病；RT-PCR；预后 Expression of novel antigen gene MLAA-34 in patients with acute monocytic leukemia and its clinical significance ZHAO Jianqiang, ZHANG Wanggang, HE Aili, ZHANG Wenjuan, GU Liufang Department of Hematology, the Second Affiliated hospital，Medicine School of Xi’an Jiaotong University, Xi’an 710004, China Abstract ： Objective The aim of this study was to investigate the expression levels of novel leukemia antigen gene MLAA-34 in newly diagnosed patients with acute monocytic leukemia and their correlation with clinical features and prognosis. Methods MLAA-34 mRNA level were detected in bone marrow samples from thirty-five AML-M5 patients, forty non-M5 AML patients, twelve patients with nonmalignant hematologic diseases and in PBMCs from ten healthy volunteers by TaqMan-based real-time quantitative RT-PCR method, And statistical analysis. Results MLAA-34 mRNA expression levels were higher in patients with AML-M5 when compared with those patients with non-M5 other subtype AML and normal control, there was a significant difference (p 0.05). MLAA-34 mRNA expression level decreased significantly in complete remission (CR) patients