珠子参水提物预处理对小鼠脑缺血再灌注损伤的影响.docVIP

PAGE PAGE 11 珠子参水提物预处理对小鼠脑缺血再灌注损伤的影响[作者简介] [作者简介] 贺海波，电话：（0717）6396558, E-mail：shmq0212@126.com 石孟琼2, 贺海波1, 覃宁玲2, 陈述威2 （443002 湖北 宜昌，三峡大学医学院：中药药理实验室1，基础医学实验中心2） [摘要] 目的 研究珠子参水提物预处理对小鼠局灶性脑缺血再灌注损伤的保护作用。方法 珠子参水提物灌胃给药7 d后制作大鼠大脑中动脉闭塞模型，缺血2 h再灌注24 h后，观察进行神经症状评分、斜板试验, 并记录小鼠的最终生存率，取大脑并用2 ,3 ,5-三苯基氯化四氮唑染色后测定梗死面积和脑含水量；制作脑匀浆测超氧化物歧化酶（SOD)、谷胱甘肽过氧化物酶（GSH-PX)、过氧化氢酶（CAT)、黄嘌呤氧化酶（XOD)酶活性和丙二醛（MDA) 含量。结果 珠子参水提物中、高剂量组 (5.0、10.0g/kg)能显著提高脑缺血再灌注小鼠成活率，明显改善缺血再灌注后的神经症状, 降低脑梗死面积和脑含水量，并能明显增加SOD、GSH-PX、CAT酶活性，降低XOD酶活性和MDA含量。结论 珠子参水提物预处理对小鼠局灶性脑缺血再灌注损伤具有明显的保护作用。 [关键词] 珠子参；局灶性脑缺血；再灌注；保护作用 [中图法分类号] [文献标志码] A Effects of water extractive of Panacis Majoris Rhizoma preconditioning on focal cerebral ischemia-reperfusion injury in mice Shi mengqiong2, He Haibo1, Qing Ningling2, Chen shuwei2 （1Laboratory of Pharmacology on Traditional Chinese Medicine, 2Experimental Center of Basic Medical Science College, China Three Gorges University, Yichang, 443002 ，China） [Abstract] Objective To investigate the protective effects of water extractive of Panacis majoris rhizoma preconditioning on focal cerebral ischemia-reperfusion (I/R) injury in mice. Methods The mice model of middle cerebral artery occlusion (MCAO) was induced, and neurological deficit scoring and the inclined board testing were performed within 24h after I/R, the The survival rate, the infarct volume and the neuron density were assessed 24h after I/R, the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT), malondialdehyde (MDA), xanthine oxidase (XOD) were also measured. Results: Water extractive of Panacis majoris rhizoma (5.0, 10.0g/kg) obviously increased the survival rate; significantly reduced the neurological deficits, infarct size and water content of focal cerebral I/R injury in mice; in brain tissue bomogenat, water extractive of Panacis majoris rhizoma at5.0, 10.0g/kg could remarkably improved the activities of SOD, GGSH-PX and CAT, and MDA, XOD content were significantly decrea