苎麻脱胶共生菌群RAMCD407中两株优势菌的分离鉴定和脱胶能力.PDF

中国农业科技导报,2013,15(4):144-150 Journal of Agricultural Science and Technology 苎麻脱胶共生菌群RAMCD407 中两株优势菌的 分离、鉴定和脱胶能力分析 ∗ 余　 苗,　 陈洪高 ,　 王超群,　 施　 薇 (武汉纺织大学纺织印染清洁生产教育部工程研究中心,武汉430073) 摘　 要:为了从苎麻脱胶共生菌群RAMCD407 中分离高效脱胶菌种,利用苎麻发酵物培养基分离筛选高效脱 胶菌株;形态、生化结合16SrDNA分子方法鉴定菌株;水解圈法结合酶活力测定验证入选菌株产酶能力;苎麻 脱胶试验核实入选菌株脱胶能力;分离出K30 和L11两个菌株。 K30 和L11都能在果胶和木聚糖平板上形成 水解圈,K30 的果胶酶和木聚糖酶活力分别为238.47 U/ mL和98.36 U/ mL,L11的果胶酶和木聚糖酶活力分 别为 170.79 U/ mL 和 64. 11 U/ mL;K30 和 L11进行苎麻生物脱胶时,能使纤维残胶率由32.3%下降为 12．43%和15.70%,纤维强力均超过5.5 cN/ dtex。 VITEK系统生化鉴定和16S rDNA序列分析都一致表明, K30与枯草芽孢杆菌Bacillus subtilis相似性高达99%,L11与蜡样芽胞杆菌Bacillus cereus相似性达到99%。 关键词:苎麻脱胶;共生菌群;芽孢杆菌;分离;鉴定 doi:10.3969/ j.issn.1008-0864.2013.04.22 中图分类号:Q935　 　 　 文献标识码:A　 　 　 文章编号:1008-0864(2013)04-0144-07 Isolation and Characterization of Two Predominant Strains from Ramie Degumming Bacterial Consortium RAMCD407 ∗ YU Miao,CHEN Hong-gao ,WANG Chao-qun,SHI Wei (Engineering Research Center for Clean Production of Textile Dyeing and Printing, Ministry of Education,Wuhan Textile University,Wuhan430073,China) Abstract:In order toisolatehighefficientdegummingstrainsfromaramierettingbacterialconsortiumRAMCD407,a specific solid medium mainly consisted of ramie gum materialsfrom ramie retting liquidsby RAMCD407 wasutilized for strain screening and isolating. Biochemical methodscombinedwith 16SrRNA gene sequencing andmorphological discrimination were chosen for strain identification. Enzyme activity analysis and practical degumming experiments were adopted to test the potential of selected strains in ramie degumming industries. Two strains designated K30 and L11were obtai