人骨髓间充质干细但胞成脂诱导方法的优化及其在探索再生障碍性贫血发病机制中的应用-内科学专业毕业论文.docx

中国医学科学院北京协和医学院硕士研究生学位论文导表达更多的PPAR3蛋白，但PPARf蛋白表达量并不随成脂时间延长持续增加。在 中国医学科学院北京协和医学院硕士研究生学位论文 导表达更多的PPAR3蛋白，但PPARf蛋白表达量并不随成脂时间延长持续增加。在 DIMR组中PPAR Y表达量并不随成脂诱导时间逐渐增加。 结论：(1)不同成脂诱导体系下，人骨髓间充质干细胞表现出不同的成脂特性。(2) DIMR培养方法有更高的成脂效率，诱导7天即可观察到脂滴形成，可以此作为成 脂诱导的观测时间点。(3)在DIMR组中PPAR),蛋白表达量不能成为检测成脂诱导 效果的良好指标。 关键词：人骨髓间充质干细胞，成脂诱导，PPAR), 2 万方数据 中国医学科学院北京协和医学院硕士研究生学位论文Part 中国医学科学院北京协和医学院硕士研究生学位论文 Part 0ne Optimization of the culture method to induce adipocyte differentiation of human bone marrow mesenchymal stem cells Abstract Background：Mesenchymal stem cells(MSCs)are multipotential stem cells with the capacity for self-renewal and multilineage differentiation,which Can be isolated from various tissues，such as bone marrow,umbilical cord，umbilical cord blood，placental and SO on．These cells have emerged as powerful tools in tissue engineering and regeneration, because they have strong capacity for expansion and differentiation into multiple cell types like osteoblasts，fat cells， chondrocytes，et．Different sources of MSCs have different biological characteristics．So they are usually used in different fields when applied to basic and clinical research．Bone marrow mesench)rmal stem cells(BM—MSCs) are easily to differentiate into adipocytes，which makes they the best candidate for adipose tissue regeneration research．From clinical standpoint，soft tissue damaged patients are afflicted with tissue resorption．For example，bum patients and HIV patients often suffer from soft tissue atrophy，which severely impact the quality of life． Depending on the resources and application,the culture methods to induce adipocyte differentiation of BM—MSCs difier from one another．This kind of situation greatly hinders the research of adipocyte differentiation of BM．MSCs，become of the lack of interpublication comparability．At the sometime，the current culture system take a lot of time and money，SO it is necessary to optimize the adipocyte differentiation culture method．This research aim at comparing the adipogenic feat