医学毕业大气污染物对大鼠肺炎性细胞因子影响.docxVIP

XX大学 毕业论文 大气污染物对大鼠肺炎性细胞因子影响 2014年6月25日 【关键词】，大气污染物；支气管肺泡灌洗液；细胞因子 摘要：目的 研究犬气污染物对大鼠肺炎性细胞因子的影响，探讨大气 污染对呼吸系统炎性损伤的机制。方法 48只Wistar大鼠，体重200?240g, 随机分为实验组(Id组、7d组和30d组)和对照组。实验组分别进行可吸入颗粒 物(PM10)染尘，SO2、NO2和CO染毒。PM10染尘采用气管注入法，灌注lml 含15mg PM10生理盐水混悬液；染毒采用静态吸入SO2、NO2、CO空气混合气， 浓度分别为15, 12, 400mg/m3,每天吸入2h,分别吸入1, 7及30d；对照组大 鼠气管注入lml生理盐水并吸入正常空气。分别于染毒后1, 7, 30d进行支气管 肺泡灌洗，观察灌洗液中口细胞总数及其分类；采用ELISA法测定支气管肺泡 灌洗液(BALF)屮炎性细胞因子水平。结果 实验组大鼠BALF屮，白细胞总数、 中性粒细胞百分比明显升高，与对照组比较差异有统计学意义(P005或P 001)o Id组大鼠BALF中，肿瘤坏死因子a(TNFot)、白细胞介素6(IL6)、白细胞 介素8(IL8)及白细胞介素10(IL10)水平均明显高于对照组(P005),各炎性细胞 因子间有较好的相关性。结论大气污染物可引起大鼠肺促炎细胞因子与抗炎 细胞因子表达失衡，造成肺组织的炎性损伤。 关键词：大气污染物；支气管肺泡灌洗液；细胞因子 Effects of air pollutants on pulmonary inflammatory cytokines in rats Abstract： Objective To study the effects of air pollutants on pulmonary inflammatory cytokines in rats,and to explore the mechanism of air pollution on inflammatory injury of respiratory system.Methods Fourty-eight Wistar rats (200~240g) were randomly divided into treated groups (ld,7d,and 30d groups) and control group.The tested rats were respectively exposed to PM10 of 15mg in lml saline by intratracheal instillation,and then were exposed to mixed gases of SO2,NO2,and CO (15,12,and 400mg/m3 respectively) by static inhalation 2h per day for ldjd and 30d respectively.One ml saline was instilled and general air was inhaled by rats of the control group.Results The counts of white blood cell (WBC) and the percentages of neutrophil (Neu) in treated groups were significantly higher than those in the control group (P005 or P001).The levels of TNFa, IL6,IL8,and IL10 in rats of oneday group were significantly higher than those in the control group (P 005).There were positive relationships among cytokines.Conclusion Air pollutants can induce the imbalance on the expression of proand antiinflammatory cytokines and result in inflammatory injury of respiratory system. Key words： air pollutants;bronchoalveolar lavage fluids (BALF);cytokines 大气污染直接作用的靶器官是呼吸系统，长期反复接触会使人体免疫力低 下，引起气管炎、支气管哮