揉髌手法对兔膝关节软骨细胞凋亡基因表达影响的实验分析.doc

The Influence Of The Rou-Bin Manipulation On Knee Joint Chondrocytes Gene Expression Of Apoptosis In Rabbit Abstract Objective: To observe the Rou-Bin Manipulation on apoptosis of knee joint chondrocytes in rabbit explore the influence of manipulation mechanisms. Methods: 50 New Zealand rabbits divided randomly into five groups: Manipulation group drug group Model group dummy-model group and the normal group 10 rabbits in each group; the first 3 groups cause IOH-model by operative dummy-model group operate without damage the corresponding vessel no treatment for the normal group.17 times of Rou-Bin manipulation on each rabbit in manipulation group the rabbit injection with sodium hyaluronate 5 times in drug group no special treatment for other rabbits． 8 weeks after modeled to take all the rabbit right knee medial tibia plateau cartilage tissue processing detected chondrocyte apoptotic gene Bcl－2、Bax、Fas expression by using immunohistochemical method statistics processing． Results: At the end of 8 weeks after being modeled, t he medial tibia pla teau cartilage e tissue o f right knee was taken for transmission elect ron microscope observation. T here was no significant change of nucleus in manipulation group and drug group, and collage nous fiber was dist rebutted in order. While in model group, Kari piano sis was presented in most cells, with or gazelles displayed unclear and collage keno us fiber disordered. No significant change for nucleus in sham model group and 4 normal group, and collage nous fiber w as distributed in order. Bcl－2 expression result(34.20±12.99)% for manipulation group (19.00±5.34)% for model group( P＜0.05 to compare with both groups) ; Bax expression result(5.94±4.58)% for manipulation group (35.88±26.35)% for model group( P＜0. 01 to compare with both groups) ; Fas expression result(13.57±11.80) for manipulation group (49.31±24.32) %for model group( P ＜ 0. 01to compare with both groups) ． Conclusion: The Rou-Bin manipulation reduced apoptosis