气流在气道上皮再生中的作用及预防移植后闭塞性气道疾病的意义-心胸外科学专业论文.pdf.docxVIP

气流在气邋上皮再生中的作用及预防移植后闭塞性气道疾病的意义 气流在气邋上皮再生中的作用及预防移植后闭塞性气道疾病的意义 英文摘要 The effect of airflow in re-·epithelization in preventing 0bliterative al刑ay disease associated with p。st鼍1．嚣nspla矗专a鼍ion Abstract Objective To explore the effect of airflow in re—epithelization of tracheal allografts with recipient—dfived mucous mem．brane in prevention of obliterative airway disease after airway transplantation。 Method Obtaining C57BL／6 mouse’s tracheal as donor,BALB／c mouse’S tracheal as recipient．The increase airflow group：the two donor tracheal were sliver in the membrane and to sew a large trachea．The decrease group：the tWO donor tracheal were attached in parallel，7 line were knotted in each ends to block up one of the donor,the other tracheal as a ventilate tracheal．control group：allografts were orthotopically transplanted into major histocompatibility mismatched BALB／c mouse(recipient)．Allografts were removed on 28 day after transplantation for histology, computerized morphometry, immunohistochemical detection of the phenotype of epithelium and ELISA detection the level ofthe IL-10、TG卜13、TNF—a．Scanning electron microscopy observe the density of the cilia．transmission electron microscopy observe the ultramicro structure of the cell。 Results increasing airflow group allografts exhibited integrated mucous membrane and ciliated cuboida,with rare fibrous tissue and lymphocyte．Decreasing airflow group exhibited lower ciliated cuboida than increasing airflOW group wim abundant fibrous tissue and lymphocyte．The ciliary coverage of increasing airflow group allogrofts Was much more than that of Decreasing airflow group and control group allografts． (p=0．000，0．008)，but similar the normal tracheaI(p=0．083)．Decreasing airflow group allografts the fibrous tissue and fibroblasts of the lamina propria Was much more than that of the increasing airflow group 020．ooo)．Immunohistochemical analysis demonstrated that all of the tracheal allografts epithelium expressed recipient phenotyo