食物过敏原胶体金免疫层析检测试纸条的研制-食品工程专业论文.docxVIP

ABSTRACTThree ABSTRACT Three kinds of colloidal gold immunochromatograph assay(GICA)for the detection of ovotransferrin from egg，almond allergen protein，and peanut allergen protein were developed．The method is simple，rapid，economic，sensitive，specific，and results in detection with the naked eye and no cross-activity with other kinds of allergen．This study first reported a GICA method for the simultaneous determination of almond and peanut in foods and it can save more time to reducing the workload in the future． Developing ovotransferrin colloidal gold immunechromatography test strip detection method，the partical size of colloidal gold is 20 nln to labeled antibody；the best pH value of colloidal gold label antibody is 8．5，the amount of antibody is 25 gg／mL；capture antibody concentration is 1．3 mg／mL；second antibody and the nano—colloidal gold-antibody probe was diluted to 80 and 5 fold；choose Millipore HF 1 35s models of nitrocellulose membrane as solid phase cartier；the article diluent selection pH 8．5 PBST buffer；O．0 1％casein diluted with PBS as block processing fluid and blocking 1 h to the NC membrane；colloidal gold treated with O．5％Tween一20．This method Can be measured for ovotransferrin standard of detection limit is 1 5 ng／mL． Developing almond allergen GICA test strip detection method，the best pH value of colloidal gold label antibodies is 9．0，the amount of antibody is 36 p．g／mL；capture antibody concentration is 1．5 mg／mL；second antibody and the nano—colloidal gold-antibody probe was diluted to 200 and 6 fold；2％BSA diluted而t11 PBS as block processing fluid and blocking 1 h to the NC membrane；other working conditions with ovotransferrin GICA test strips in the same way．This method Can be measured for almond protein and standard of detection limit is 1 00 ng／mL． Developing peanut allergen GICA test strip detection method，the best pH value of colloidal gold label antibodies is 9．0，the amount of antibody is 25 pg／mL；capture antibody concentration is 0．5 m