加拿大披碱草与披碱草圆柱披碱草2个种间杂种F1及其亲本的AFLP.PDF

16 5 草 地 学 报 2008 9 Vol. 16 No.5 ACTA AGRESTIA SINICA Sep. 2008 2 F AFLP 1 于 卓, 马艳红, 李小雷, 周亚星, 李晓宇, 李造哲 ( , 010019) : ( E ly mus canadens is L. ) ( E. dahur icus Turcz.) ( E. cy lindr icus ( ranch.) Honda) 2 1 , A LP , 2 1 , : 13 1247 A LP , 1042 , 83. 56% ; ( GD) 0.4276~ 0.7486 , GD 0.65 , 5 2 , 1 @ , 2 @ 1 1 : ; ; ; ; A LP 1 : Q943 :A : 1007-0435( 2008) 05-0431-06 AFLP Analysis on Interspecific Hybrid F1 and the Parents between Ely mus canadensis L. and E. dahuricus Turcz. or E. cy lindricus ( Franch. ) Honda YU Zhuo, MA Yan-hong, LI Xiao-lei, ZHOU Ya-xing, LI Xiao-yu, LI Zao-zhe ( College of Agr onomy, Inner Mongolia Ag ricultural University, Hohhot, Inner Mongolia Autonom ous Region 010019, China) Abstract In order to provide a molecular basis for identification of hybrids and breeding of progenies, the genetic difference of Ely mus canadensis L. @ E. d ahur icus Turcz. hybrid 1,E . canadensis @ E. cy lindricus ( ranch.) Honda hybrid 1 and their parents were compared and analyzed by A LP molecular marker technique. The results show that 1247 loci of A LP markers were amplified with 13 suitable primer comb-i nations, and 1042 loci among which were polymorphic and the percentage of polymorphism loci reached to 83. 56%. The genetic distance of test plants was ranged from 0.4276 to 0.7486.T aking the genetic distance value of 0. 65 as threshold, 5 test plants were clustered into 2 groups:the first group included E. canaden- sis,E. cy lindricus, and E.