大豆DGAT基因家族的鉴定和表达分析.PDF

中国农学通报 2013,29(12):55-61 Chinese Agricultural Science Bulletin 大豆DGAT 基因家族的鉴定和表达分析 刘贵芹，邵 群，黄荣峰，冯献忠 （山东省高校系统生物学重点实验室/ 山东师范大学生命科学学院，济南250014 ） 摘 要：旨在利用生物信息学方法鉴定大豆DGAT 基因，为其功能研究奠定基础。通过生物信息学的方 法，在全基因组水平上对大豆DGAT 基因家族进行了鉴定和分类，并分析了其基因结构、蛋白质结构域 特征、系统发生及基因表达方式。结果表明：大豆中存在10 个DGAT 家族基因，分别属于DGAT 1、 DGAT2、DGAT3 亚家族，分布在6 条染色体上，其中DGAT 1 亚家族基因主要在茎端分生组织和绿色果 荚中表达；DGAT3 亚家族基因主要在根中表达；而DGAT2 亚家族基因表达范围最广，主要在根瘤、叶、 花和绿色果荚中表达。大豆DGAT 家族基因在结构及表达上的差异揭示了其功能的多样性及大豆脂肪 酸代谢的复杂性，将为研究大豆的脂肪酸代谢途径提供重要的信息。 关键词：脂肪酸合成；DGAT 基因家族；基因结构；基因表达 中图分类号：S565.1 文献标志码：A 论文编号：2012-4153 Characterization and Expression Analysis of DGAT Gene Family in Soybean Liu Guiqin, Shao Qun, Huang Rongfeng, Feng Xianzhong (KeyLaboratoryofSystemsBiologyinUniversitiesofShandong/CollegeofLifeScience, ShandongNormalUniversity, Jinan 250014) Abstract: The aim was to identify the DGAT gene in soybean with bioinformatics methods, and provide the basis for studying its function. In this study, DGAT family genes of soybean were characterized in genome wide. Their gene structures and secondary structures of proteins, as well as phylogeny and expression pattern were also investigated. Phylogenetic analysis characterized 3 classes of DGAT genes in soybean, which are belonged to DGAT 1, DGAT2 and DGAT3, and they distribute in 6 chromosomes of soybean genome. Whole genome expression analysis revealed that DGAT 1 subfamily genes mainly expressed in SAM and green pod while DGAT3 subfamily genes mainly in root, DGAT2 family genes broadly expressed in nodule, leaf, flower and green pod. The difference of DGAT gene structures and expression patterns suggested that