hfhg5基因的功能研究遗传学专业论文.docxVIP

ABSTRACTAccording ABSTRACT According to the latest statistics of world health organization，heart disease mortality is in the first place of death by 1 2．9％．As a need with long-term and in—depth study of the disease，heart disease has become the world’S problems which scientists are eager to solve．It is well known that the early symptoms of heart disease is pathological myocardial hypertrophy． In order to achieve to cure heart disease fundamentally,the regulating mechanism of myocardial hypertrophy was systematically studied at the molecular level． Existing research showed that HFHG5 were found with high expression in mice model(the aorta banding method)and the specimens of patient tissue，which suggesting that the occurrence of myocardial hypertrophy might be associated with HFHG5．With the analysis of Luciferase report system and CO—IP experiment，researchers found that HFHG5 was associated with TGF-13／Smads signaling pathway and HFHG5 actually interact with Smad3／4，which suggesting that HFHG5 was likely to be involved in the TGF—B／Smads signaling pathway．But the detail of the link between TGF-B signaling pathways and HFHG5 or the regulating mechanism of myocardial hypertrophy had not been studied，on which it has begun to research． In order to study the mechanism of HFHG5 gene regulating myocardial hypertrophy occurrence，this paper had carded out the following research： III 万方数据 (1)In (1)In order to facilitate the development of the experiment，it needed to construct the recombinant adenovirus vector pAd-HFHG5 and prepare the adenovirus，t11at adenovirus Ad-HFHG5 was verified to construct successfully．Ad-HFHG5 was mainly used for the experiment of primary myocardial cells of neonatal rat with the high expression of HFHG5．At the same time，we successfully built a interference vector pSuper-HFHG5- shRNA and verified the existing expression vector pCMV-Myc-h-HFHG5 from the laboratory．This pair of carder was mainly used for interference and high expression of HFHG5