大豆抗病毒基因gmnh23的筛选鉴定及功能分析生物化学与分子生物学专业论文.docxVIP

GmNH23的表达，最终达到成功侵染和扩增。我们的结果揭示了 GmNH23的表达，最终达到成功侵染和扩增。我们的结果揭示了 miR2／9．GmNH23的调控关系及其在SMV侵染中的作用，为进一步阐 明SMV-大豆互作的分子机制打下了基础。 关键词：SMV；NBS．LRR蛋白；超敏反应(腿)；GmNH23；miR2／9 万方数据 内蒙古大学硕士论文Ⅱ)EN田匝ICATION 内蒙古大学硕士论文 Ⅱ)EN田匝ICATION AND FUNCTIONAI，STUDY 0F AⅥRUS． RESISTANCE GENE GmA强陀3 FROM SOYBEAN Soybean Mosaic Virus(SMV)is a plant virus that infects soybean worldwide，leading to serious yield reductions or even crop failure．While the progress has been made on the study of molecular marker and QTL mapping about SMV resistance gene(R)，the gene itself still has not been found．N protein is a NB S—LRR family TMV-resistant protein found in tobacco．35 homologs ofN gene were found on soybean chromosome and were named GmNH(G__lycine max丛函molog)． Overexpression of an R gene and subsequent pmhogen infection normally cause hypersensitive response fliP,)，a phenomenon of programmed cell death，which is useful for screening and identification of R gene．In this study,we investigated the resistance of 23 GmNH genes toward SMV using this hypersensitive response．We also evaluated the resistance of GmNH genes toward TMV as t11ey liFe the homolog of TMV-resistant N gene．GmNH23，an N． homolog in soybean，was found to be resistant to both SMV and叫 while the others showed resistance to neither SMⅣnor TAnf Bioinformatic analysis showed that GmNH23 was predicted target ofmiR- 2 and miR-9，which Was verified by both RLM-RACE and dual luciferase 万方数据 内蒙古大学硕士论文assay．GmNH23 内蒙古大学硕士论文 assay．GmNH23 was down—regulated when successful infection is established after longer infection of SMV,while the level of miR一2 and miR一9 was up-regulated at this stage，suggesting a correlative expression of miR-2／9 and their target gene GmNH23．Taken together,GmNH23 mediates soybean resistance to SMV,and SMV antagonizes this resistance by upregulating the level of miR-2／9．Our results demonstrated the regulatory roles of miR2／9 on GmNH23 and its involvement in SMV infection，which is important for further illustration of the molecular mec