东北黑土不同土壤利用方式下微生物群落的变化特征农业资源与环境土壤学专业论文.docxVIP

Coh阿】TSCoNTENTS Coh阿】TS CoNTENTS Abstract in Chinese ．1 Abstract in English． ．．．．．．．．．．．．．．．．．．． ．．．． ． ． ．．．． ．．．．．． ．．III 1 Introduction ． ．．1 1．1 Research background ．．1 1．2 The research purpose and meaning l 1．3 Research progress at home and abroad 2 1．3．1 7Ille role ofmicroorganisms in soil ecosystem 2 1．3．2 Soil microbial community diversity ofresearch methods ．．3 1．3．3 In soil microbial change under different soil using the way ofrelated research ．．5 1．4 The research content and the technical route 5 1．4．1 The research content 5 1．4．2rechnical route ． ．6 ：}Materials and Methods ． ． ．．7 2．1 The study area ． 7 2．1．1 Tlle northeast black earth area 7 2．1．2 Test geographical location and climate and hydrological conditions 7 2．2 TESt setting ． ．．．8 2．!；1rhe tested soil ．8 2．4THeresearchcontentandthemethodandsteps．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．9 2．4．1 Against determination ofthe number of soil culturable microorganisms 9 2．4．2Soil microbial community diversity Biolog microplate method ．10 2．4．3 Soil DNA extraction and quantitative PCR analysis ．．11 2．4．4 454 high-throughput sequencing technologies ．12 3 The results and analysis ．13 3．1 Different soil culturable microbial quantity using way ．13 3．2 Different soil using Biolog analysis results 14 3．3 Soilmicrobial communityOiltheusing characteristicofallkinds ofcarbon source 16 3．4 Quantitative PCR results ．17 3．4．1 Track 16 bacteria and archaea 16 biomass variation characteristics 17 3．4．2 Ammonia oxidation bacteria and ammonia oxidation archaea biomass variation characteristics ． ． ．．17 3．5 454 hi曲-throughput sequencing classification results 18 3．5．1 Track ofdoor(phylum】level classification 18 3．5．2 Microbial community diversity index ．．20 3．5．3 Microbial community structure change 22 4 Discussion ． ．：!zI IIl 万方数据 东北农业大学农学硕士学位论文!；IConclusion 东北农业大学农学硕士学位论文 !；IConclusion ． ． ． ． ．．． ． ． ． ． ． ． ． ． ． ． ． ． ． ．．． ． ． ．．． ． ． ． ． ． ． ． ．．：16 Acknowledgement． ． ． ． ．．． ． ． ． ． ．． ．． ． ．．． ． ． ．