次郎柿与上西早生柿acc氧化酶的转基因研究食品科学专业论文.docxVIP

2．1 2．1 Studying effects of antibiotic on the regeneration of Uenishiwase leaves in vitro and the proper concentration of antibiotic had been found． Studying effects of antibiotic on the regeneration of Uenishiwase leaves and leaf explants，ensured the proper concentration of antibiotic．Using 200 mg／L Cef and 30 mg／L Sp on growth of resistant shoots． 2．2 The transgenic technic of Uenishiwase Cutting 4rnmx4 innl from the base of 2 to 3 leaves of the top of Uenishiwase of 4．5 years in vitro from generation to generation，putting with top of the back of leaf on the medium of DKW(1／2N)+ZT 1．0 merlE+TDZ 0．5 mg／L，dipping the leaves after pre—cultured in darkness for three days into Agrobacterium suspension which was AS 1 00 mg／L in it fOr ten minutes．transferring the leaves to the medium of DKW(1／2N)+ZT 1．0 merlE+TDZ 0．5 mg／L with CO—cultured in darkness for four days，then diverting the leaves to the medium of DKW(1／2N)+ZT 1．0 medL+TDZ 0．5 mg／L+Cef 200 mg／L witIl de．cultured in darkness for three days，finally transferring the leaves to the medium of DKW(1／2N)+ZT 1．0 medL+TDZ 0．5 mg／L+Cef 200 mg／L+Sp 30 mg／L for selecting in light，once every twenty—five days，transferring the leaves to the medium of DKW+ZT 1．0 mg／L+Sp 30 mg／L+Cef 200 mg／L after seven to eight times． In this study,we obtained four Uenhiwase transgenetic plants carrying ACC oxidase RNAi gene which identified by histochemical analysis of GUS activity and PCR。 Key words：Jiro；Uenishiwase；Agrobacterium—mediated；Genetic Transformation． 缩略词表-—__-—●----———-●●_-●__——-__●—●——__●_-__-—●__-●_-—●__-●--●●---●-___--_-___I-_-____-__—————_———_——————————————一 缩略词表 -—__-—●----———-●●_-●__——-__●—●——__●_-__-—●__-●_-—●__-●--●●---●-___--_-___I-_-____-__—————_———_——————————————一一缩略塑 茎塞全整 主茎全整 Abbreviate English Chinese ACC l-Aminocyclopropane．1．Carboxylic．Acid 1·氨基-1·羧基环丙烷 ACO ACC Oxidase ACC氧化酶 ACS ACC Synthase ACC合成酶 AS Acetosyringone 乙酰丁香酮 A．t Agrobacterium tumefaciens 农杆菌 bp Base pair 碱基对 Cef Cefotaxime 头孢霉素 cDNA complementary DN