北疆苜蓿根部侵染病害病原菌分子检测植物保护专业论文.docxVIP

Molecular Molecular Detection of Pathogens ofAlfalfa Root Diseases in North—Xinjiang Abstract 』蛆falfa is the most imDortant planting forage grass in Xinjiang．A1falfa is of the maln diseases in A1falfa production．A1falfa verticillium wilt is potential threat for the Alfalfa production．This study adopts the specific primer PCR detection technology inspect the mixed diseased sample located in the main Alfalfa planting regions of north Xinjiang and the diseased sample collected per single plant in the single serious illness field，as well as the regularly collected diseased sample of multiple germs after the mixed inoculation respectively．In order def_me the pathogenic species of development in nortbem region of Xinjiang,various pathogenic fungus infection dynamics Alfalfa and lay the foundation of research the pathogens compound infcction regular and control technique；meanwhile．carry PCR detection the extensively collected diseased samples veilfy Alfalfa verticillium wilt in the development region of northern xinjiang；Provide the basis for the effective prevention and control ofthe disease． The specificity lcad PCR detection of mixed diseased samples ofAlfalfa and basal stem shows that：there are 3 species of FusarJum in diseased samples(Fusarium equiseti,Fusarium acuminatum and Fusarium oxysporum)，and 1 species of Rhizocton记(Rhizoctonia Solani)；only 3 species of FHsarium tFusarium．acuminatum?Fusarium．solani and Fusarium oxysporum、are detected from stem base diseased samples；which give血e fact that there 4 mffm Fusarium species(F equiseti,F acuminatum，F solani and F oxysporum)and 1 Rhizoctonia species僻．Solani)of pathogenic species in the main Alfalfa planting regions of north Xinjiang and there is slight different between the reot and basal stem r足80lani and E equiseti only detected in the diseased samples．and f solani only detected in the basal stem diseased samplesl． The specificity lead PCR detection of 15 disease samples collected per single plant in the single serio