《SNT1197-2003-油菜籽中转基因成分定性PCR检测方法》.pdfVIP

Protocol of the polymerase chain reaction for detecting genetically modified components in rapeseeds PCR @WA. *6@SHT9ttNiPCDfB l , % t TMR@4.@B;l;Bff%!fdJle##fsel@BPCR Me TPI*%.5!XW@B8BSHT*4%?Bo 3. 1 t)S transgene ~ ~ # ; Q ~ i f ~ a ~ , * aDNA~ ~B ~i I~. ~~ ~~mN +aAr +nH , @ ~ B P ~ # + ~ Ei-S.Q1Yi,U@BalfJbR4~8ii#611jPP%~e 3 2 firL#*K polymerase chain reaction, PCW #SE4@315kS2f!k%J%6%,fiDNA #$B#H#SaMEj%#T,B#%9JR it~RCll$hJJ)41]-4#DNA m@k#flff~-BIjllj:.@3ilJ%%aIkThiX@ DNA Se -m*,@BrMmsm%Bunfi%arme 3 3 a#i% 3.3. 1 PCR; polymeresc chain react~on,iQ-% PCR. 3. 3 . 2 DNArdeoxyribonucleic ~ c i d , a # % # l @ ~ 3.3.3 dNTP;deoxyribonucleos~detripl1osihate,A#RBBZbf9@. 3 . 3 . 4 dATP, deoxyadenos~netriphosphatc,8#$lMfi 7@i!#ie 3 . 3 . 5 dCTPIdeoxycytid~netr~phosphnte,H##H%~.ljlPaQ. 3 . 3 . 6 dCTPtdeoxyguanos~netr1phos~hatc,A#$l4%3@@. 3 . 3 . 7 dTTP; deoxythymidine triphospllate,A#~!jQH#:a(ft. 3 . 3 8 dUTPldeoxyuridine tripho~phatc,R#$t@-ffZ7#@!~ 3.3.9 UDGIuracil DNA glycosylase,!3!@/@ DNA-%#@. 3.3.10 bp Ibase pair, @XQ. 3 3. 11 EDTAleth~lenediaminetetraacetic acid, f@fl@41. 3.3. 12 Tag I TaqDNA Polymerase, DNA SeIBB), 3.3.13 TEITris-HC1,EDTA $@#%. 3.3.14 SDS~sodiumd o d e c y l s ~ l f a t e , + = ~ ~ ~ @ ~ ~ 3.3.15 Tris; tris (hydroxymethyl) aminomethanevZ(BtB#)$2$;Sm 3.3. 16 PEP I pho~phoenolpyruvatecarboxylase gene ,?i@l@#lBSWRm%.cI:@. 3 3. 17 FMV 35S135Spromoter from a modified figwort mosaic virus(caulimovirus group) -k;,$a Rt.%f* 35s B 3 3 . 3.3. 18 CP4 EPSPSl 5-enolpyruvylshikimate3-phosphatesynthase gene, 5-% Q: @-3-@ @ fi €%I. 3 3. 19 CTPIchloroplast transit peptide gene, Utb3#BM!lkS F4a 3.3.20 G0X:glyphosate oxidoreductase generWE8P)tSfi%RWSH