乙草胺降解芽孢菌的分离及降解效果研究微生物与生化药学专业论文.docxVIP

全氮、有效磷和有效钾的破坏；施用降解菌液后，相比空白组，小麦地上部分增高 全氮、有效磷和有效钾的破坏；施用降解菌液后，相比空白组，小麦地上部分增高 1 17％，总鲜重增重56％，根长增长27％，表明菌株L3对污染土壤中敏感作物小麦 的生长修复具有良好的效用，即该菌剂的使用可减轻因乙草胺残留所造成的下茬作 物潜在危害。 5、应用平皿生化反应、薄层色谱法及分光光度法检测了菌株L3产生促生物质 的能力。结果发现，菌株L3可产生嗜铁素、吲哚乙酸和一定量的小分子有机酸， 其嗜铁素A／Ar比值为O．61，全部为羧酸型，吲哚乙酸的浓度为75．6 mg／L。证实L3 菌株在乙草胺污染土壤修复中具有多方面实际应用价值。 本研究最终分离到乙草胺高效降解菌Bacillus subtilis L3，并探明了其生长、降 解特性和实际应用效果。 关键词：乙草胺降解；枯草芽孢杆菌；降解特性；土壤修复；促生长 万方数据 The The separation ofAcetochlor-degrading spore bacteria and its degradation effect Candidate：Yan Zhiyu Advisor：Li Shu—na Major：Microbiology and Biochemical Pharmacy Abstract Acetochlor is one of the three most representative herbicides(Glyphosate， Butachlor,Acetochlor)，with a huge production and usage in our country．It is less volatile，difficult in photolysis and residual easily,besides，acetochlor has a direct toxicity, which bringing a potential problem in ecological and environmental security with its long-term vast application．Thus the research of Acetochlor’S microbial degradation has become a hotspot gradually． This study aimed at screening and identifying the efficient acetochlor-degrading bacteria from the farmland which has been used Acetochlor for a long time and in large quantities，and the biological and degrading characteristics of the strain was also studied． Further more，a preliminary study on bacteria’S microbial inoculum production and its practical application was conducted，The main research results are as follows： 1．Six acetochlor-degrading strains which Can use acetochlor as the only power source to grow were isolated from the farmland soil which have been used acetochlor in long-term and large quantities．One of them，numbered as L3，whose degradation rate was highest，was identified finally as Bacillus subtilis after morphological observation， 1 6S rDNA sequence homology analysis and physiological and biochemical experiment． The serial number of L3’S 1 6S rDNA sequence in GenBank was：KM5738 1 6． 2．The growth curve of L3 was draw by its growth characterist