《SNT1155-2002-植物检疫玉米霜霉病菌检疫鉴定方法》.pdfVIP

中华人民共和国出入境检验检疫行业标准 SNIT 1155-2002 植 物 检 疫 玉米霜霉病菌检疫鉴定方法 Plantquarantine-Methodsforquarantine andidentificationofPeronosclerospora(lto)C.G.Shaw 2002一11一25发布 2003一05一01实施 中 华 人 民 共 和 国 发 布 国家 质 量 监 督检 验 检 疫 总 局 SN/T1155-2002 目 次 前言 ···。···················，······‘·····，···。·····················，·········，，··················，················，···一· I 1 范围 ····································，···········，······························································…… 1 2 原理 ···································································，·······························，··，·····一 1 3 仪器和用具 ·······································································，····························…… 1 4 试剂和材料 ····································································································… … 1 5 现场检疫与取样 。‘。·····，······‘····· ·········· ·，··········，·，·······，，，，，， ··，，，，·，····，，，，···…… 2 6 实验室检验 ··············，·······················，··························································，···…… 3 7 结果评定 ·····························，······························，··········，··…… ·‘···········，·…… 5 8 样品保存 ·········。··············································，················································…… 5 附录A(资料性附录) 玉米霜霉病菌寄主范围 ··························································…… 6 附录B资料性附录) DNA提取、扩增与电泳，···························，························，··… … 7 附录C(规范性附录)PCR扩增引物 .·“一 ’二’“9 附录D(资料性附录)玉米霜霉病菌卵抱子、分生抱子、分生抱子梗图 ····························…… 10 SN/T1155-2002 .几 J . .叫 .- 月Ili 昌 本标准的附录C为规范性附录，附录A、附录B、附录D为资料性附录